Journal ArticleDOI
Aspects of in vitro fertilization and embryo culture in domestic animals.
Reads0
Chats0
TLDR
An examination of the spermatozoa and oocyte incubation media showed no media or protein supplement to be superior in promoting in vitro fertilization in cattle, sheep or swine.Abstract:
Summary Various aspects of the major components of in vitro fertilization and embryo culture in domestic farm animals are discussed. An examination of the spermatozoa and oocyte incubation media showed no media or protein supplement to be superior in promoting in vitro fertilization in cattle, sheep or swine. Generally, ovulated oocytes or activated follicular oocytes were penetrated by sperm more frequently than were immature oocytes. Spermatozoa that were incubated in vivo or in the oviducts of a different species generally achieved higher oocyte penetration than did spermatozoa incubated in vitro. In most in vitro fertilization studies, an in vivo component was introduced, generally oocyte or sperm maturation, which served to confound analysis of the in vitro results. Furthermore, the relatively low level of success and high incidence of chromosomal abnormalities with in vitro fertilization in other species require that careful and complete studies analyzing the components of in vitro fertilization in domestic farm animals be conducted. The culture of embryos from domestic farm animals is detailed, with emphasis on in vitro conditions and rate of success. As with laboratory animals, bovine, ovine, porcine and caprine embyos of fewer than eight cells are more difficult to culture to the blastocystread more
Citations
More filters
Journal ArticleDOI
Development of preimplantation embryos of the golden hamster in a defined culture medium.
TL;DR: The data suggest that an important metabolic change may occur in hamster embryos during a critical period at the 8-cell stage of development, which is an approach towards achieving complete preimplantation development ofHamster embryos in vitro.
Journal ArticleDOI
In vitro development of bovine one-cell embryos: Influence of glucose, lactate, pyruvate, amino acids and vitamins
Y. Takahashi,N. L. First +1 more
TL;DR: It is demonstrated that a semi-chemically defined medium can successfully support the development of bovine embryos to the morula stage to a limited extent, but the medium lacks some nutrients or growth factors to fully support development through the blastocyst stage.
Journal ArticleDOI
Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells
Fulvio Gandolfi,R. M. Moor +1 more
TL;DR: It is concluded that during the first 3 days after fertilization cleavage will progress at a normal rate on different feeder-layers but oviduct cells appear to be required for the acquisition of full embryonic viability.
Journal ArticleDOI
Co-culture of early cattle embryos to the blastocyst stage with oviducal tissue or in conditioned medium.
W. H. Eyestone,N. L. First +1 more
TL;DR: Five-8-cell embryos from superovulated cattle were co-cultured with oviducal tissue suspended in Ham's F10 + 10% fetal calf serum or in F10FCS alone and embryos obtained from in-vitro maturation and fertilization were used to compare development between co-culture and medium conditioned by ovidUCal tissue.
Journal ArticleDOI
Normal development following in vitro fertilization in the cow.
Benjamin G. Brackett,Daniel Bousquet,Melinda L. Boice,William J. Donawick,James F. Evans,Michael A. Dressel +5 more
TL;DR: Findings suggested vigorous progressive sperm motility and acrosome integrity to be important features of good sperm samples and the first calf resulting from in vitro fertilization has been found to be completely normal.
References
More filters
Journal ArticleDOI
Plaque formation and isolation of pure lines with poliomyelitis viruses.
Renato Dulbecco,Marguerite Vogt +1 more
TL;DR: Pure virus lines were established by isolating the virus population produced in single plaques, which had the same morphological, serological, and pathogenic properties as the parent strain.
Journal ArticleDOI
Successful culture in vitro of sheep and cattle ova
TL;DR: This communication describes the successful culture of one-cell to eight-cell sheep ova and one- cell and eight- cell cattle ova to the morula and blastocyst stages and reports a high embryo survival after transfer of cultured Ova to recipient animals.
Journal ArticleDOI
Observations on the penetration of the sperm in the mammalian egg.
TL;DR: It is considered that the evidence so far put forward for the fertilization in vitro of mammalian eggs is inconclusive, and attempts made to effect the fertilizing of the mammalian egg in vitro are terminated.
Related Papers (5)
Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells
Fulvio Gandolfi,R. M. Moor +1 more
Co-culture of early cattle embryos to the blastocyst stage with oviducal tissue or in conditioned medium.
W. H. Eyestone,N. L. First +1 more