Journal ArticleDOI
Cell guidance by ultrafine topography in vitro
Reads0
Chats0
TLDR
It is clear that topography on this scale can control cell behaviour, but guidance of this type is strongly dependent on cell type and cell-cell interactions.Abstract:
Laser holography and microelectronic fabrication techniques have been employed to make grating surfaces in fused quartz with ultrafine period (260 nm) in an attempt to mimic the topography of aligned fibrillar extracellular matrix (ECM), which, in the past, has been shown to affect the behaviour of cells in vitro and in vivo. The alignment of BHK cells, MDCK cells and chick embryo cerebral neurones on 260 nm period grating surfaces (130 nm grooves separated by 130 nm) of various depths (100, 210 and 400 nm) was examined. While all gratings aligned BHK cell populations, the degree of alignment was dependent on depth. The response of single MDCK cells to the grating patterns was both to align precisely to the direction of the gratings, and to elongate; only their elongation was depth-dependent. MDCK cells that were part of epithelial cell islands, and the outgrowth of neurites from chick embryo neurones, were mainly unaffected by the grating surfaces. It is clear that topography on this scale can control cell behaviour, but guidance of this type is strongly dependent on cell type and cell-cell interactions.read more
Citations
More filters
Journal ArticleDOI
Effects of synthetic micro- and nano-structured surfaces on cell behavior
TL;DR: The fabrication of synthetic micro- and nano-structured surfaces and the effects of such textured surfaces on cell behavior are reviewed and the hypothesis that the topography of the basement membrane plays an important role in regulating cellular behavior is proposed.
Journal ArticleDOI
Topographical control of cells
TL;DR: A review of the literature on the reaction of cells to their surrounding topography and a summary of the types of prosthesis where advantage has been taken of the ability to fabricate topography.
Journal ArticleDOI
Epithelial contact guidance on well-defined micro- and nanostructured substrates
TL;DR: This work documents that biologic length-scale topographic features that model features encountered in the native basement membrane can profoundly affect epithelial cell behavior.
Journal ArticleDOI
Influence of nanoscale surface topography on protein adsorption and cellular response
TL;DR: Different methods to synthesise and fabricate surfaces with well-defined nanoscale topography are reviewed, and how such nanostructured surfaces can be used as model systems in protein and cellular assays are discussed.
Journal ArticleDOI
Modern biomaterials: a review - bulk properties and implications of surface modifications.
TL;DR: The many questions concerning the conformational form and control of bound proteins and how this may impact on cell adhesion in the first instance and later on cell signalling and implant integration can be answered by systematic investigations using model materials.