Journal ArticleDOI
Cellular mechanism of the furosemide sensitive transport system in the kidney
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TLDR
Experiments suggest that potassium ions are involved in the sodium chloride transport system because potassium reabsorption is inhibited by furosemide and because intracellular sodium falls significantly whenassium ions are removed from the tubular fluid.Abstract:
Experiments were performed in the distal tubule of the doubly-perfused kidney of Amphiuma to determine active and passive forces, involved in the transport processes of potassium, sodium and chloride. Ion-sensitive microelectrodes and conventional microelectrodes were applied to estimate intracellular ion activities, cell membrane potentials and net flux of potassium and chloride under control conditions and during inhibition of active transport. Sodium chloride cotransport, located in the luminal cell membrane is postulated, based on the following observations:read more
Citations
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Journal ArticleDOI
Functional Significance of Cell Volume Regulatory Mechanisms
Florian Lang,Gillian L. Busch,Markus Ritter,Harald Völkl,Siegfried Waldegger,Erich Gulbins,Dieter Häussinger +6 more
TL;DR: Cell volume may be considered a second message in the transmission of hormonal signals, and alterations of cell volume and volume regulatory mechanisms participate in a wide variety of cellular functions including epithelial transport, metabolism, excitation, hormone release, migration, cell proliferation, and cell death.
Journal ArticleDOI
Properties of the basolateral membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney. A model for secondary active chloride transport.
Rainer Greger,Eberhard Schlatter +1 more
TL;DR: The present data are used to draw a tentative model for the NaCl reabsorption in the cTAL segment and it is concluded that the K+ exit at the basolateral membrans is mainly electroneutral and that Cl− leaves the cell both electroneutrally (KCl) and diffusionally, which is the main source for the lumen positive transepithelialPD.
Journal ArticleDOI
Properties of the lumen membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney
Rainer Greger,Eberhard Schlatter +1 more
TL;DR: The present data indicate that an essentially complete recycling of K+ across the lumen membrane of the cortical thick ascending limb (cTAL) of rabbit nephron is feasible and is strengthened by the results of another series in which no evidence for a Cl− conductive pathway in theLumen was obtained.
Journal ArticleDOI
Distribution of immunoreactive Na+,K+-ATPase in gerbil cochlea.
Bradley A. Schulte,Joe C. Adams +1 more
TL;DR: The distribution of Na+,K+-ATPase was mapped in cochleas of mature gerbils with normal hearing, using a specific and sensitive immunocytochemical method, and three different populations of cells lying in the inferior portion of the spiral ligament and at the medial and lateral border of the scala vestibuli just superior to the attachment of Reissner's membrane were detected.
Journal ArticleDOI
Evidence for electroneutral sodium chloride cotransport in the cortical thick ascending limb of Henle's loop of rabbit kidney.
TL;DR: It is concluded that the cotransport across the luminal membrane of the cTAL segment is electroneutral and involves 1 Na+∶2 Cl−∶1K+.
References
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Journal ArticleDOI
Function of the thick ascending limb of Henle's loop.
Maurice B. Burg,Nordica Green +1 more
TL;DR: Water permeability (Lp) is low, which combined with the NaCl transport accounts for the dilution of the urine in this segment, and Na permeability is greater than Cl permeability, as indicated by radioisotope measurements of ion fluxes.
Journal ArticleDOI
Electrically silent cotransport of Na+, K+ and Cl− in ehrlich cells
TL;DR: A cotransport system for Na+, K+ and Cl- in Ehrlich cells is described and appears to function in cell volume regulatin as it tends to make the cell swell, thus counteracting the shrinking effect of the ouabain-sensitive (Na+, K+) pump.
Journal ArticleDOI
Furosemide effect on isolated perfused tubules.
TL;DR: The principal renal action of this drug is to inhibit active Cl transport in the thick ascending limb of Henle's loop with resultant decrease in net NaCl absorption and electrical PD.
Journal ArticleDOI
Sodium chloride and water transport in the medullary thick ascending limb of Henle. Evidence for active chloride transport.
Antonino S. Rocha,Juha P. Kokko +1 more
TL;DR: The medullary thick ascending limb of Henle is water impermeable while having the capacity for active outward solute transport as a consequence of an electrogenic chloride pump and the combination of these characteristics allows this segment to generate a dilute tubular fluid and participate as the principal energy source for the overall operation of the countercurrent multiplication system.
Related Papers (5)
Presence of luminal K+, a prerequisite for active NaCl transport in the cortical thick ascending limb of Henle's loop of rabbit kidney.
Rainer Greger,Eberhard Schlatter +1 more
Properties of the lumen membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney
Rainer Greger,Eberhard Schlatter +1 more