Journal ArticleDOI
Characterisation of inhibitors of acetylcholinesterase by an automated amperometric flow-injection system
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TLDR
Acetylcholinesterase was immobilised on magnetic particles and integrated in a flow-injection system via a magnetic reactor to be applied to automated determination and characterisation of enzyme inhibitors.About:
This article is published in Analytica Chimica Acta.The article was published on 1995-01-20. It has received 81 citations till now. The article focuses on the topics: Immobilized enzyme & Acetylthiocholine.read more
Citations
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Journal ArticleDOI
Review on the use of enzymes for the detection of organochlorine, organophosphate and carbamate pesticides in the environment.
TL;DR: Enzymatic methods of detection of pesticides can serve as a tool for screening large samples which can be followed up with the more traditional chromatographic methods of analysis.
Journal ArticleDOI
Superparamagnetic nanoparticles as versatile carriers and supporting materials for enzymes
TL;DR: As a very promising strategy, superparamagnetic nanoparticles based on magnetite and maghemite have been recently employed as supporting materials for enzymes, exhibiting striking characteristics, such as large surface area, mobility and high mass transference.
Journal ArticleDOI
Microfluidic Assays of Acetylcholinesterase Inhibitors
TL;DR: A microfabricated device for flow injection analysis and electrophoretic separation of acetylcholinesterase (AChE) inhibitors is described, and a Gaussian peak was observed for competitive inhibitors, whereas a broad negative peak was seen for irreversible inhibitors.
Journal ArticleDOI
Design of acetylcholinesterases for biosensor applications
TL;DR: Approaches to purify recombinant AChEs to a degree that is suitable for analytical applications will be elucidated as well as the various attempts that have been undertaken to increase the sensitivity of AChE to specified organophosphates and carbamates using side-directed mutagenesis and employing the enzyme in different assay formats.
Journal ArticleDOI
Evaluation of the use of free and immobilised acetylcholinesterase for paraoxon detection with an amperometric choline oxidase based biosensor
TL;DR: In this article, a free and immobilised acetylcholinesterase (AChE) was used for the development of an amperometric biosensor for organophosphorus (OP) insecticides based on enzyme inhibition.
References
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Journal ArticleDOI
A new and rapid colorimetric determination of acetylcholinesterase activity.
TL;DR: A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described and Kinetic constants determined by this system for erythrocyte eholinesterases are presented.
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Acetylcholinesterase: enzyme structure, reaction dynamics, and virtual transition states
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Dual electrode liquid chromatography detector for thiols and disulfides
Laura A. Allison,Ronald E. Shoup +1 more
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Sensitive detection of pesticides using amperometric sensors based on cobalt phthalocyanine-modified composite electrodes and immobilized cholinesterases
Petr Skládal,Marco Mascini +1 more
TL;DR: The determination of organophosphate and carbamate pesticides was carried out using cobalt phthalocyanine-modified carbon epoxy composite electrodes coupled with acetyl- or butyrylthiocholine as substrates and detection limits are similar as those obtained when analyses were performed using free cholinesterase and 10 min incubation with inhibitor.
Journal ArticleDOI
Sensitive detection of pesticides using a differential ISFET-based system with immobilized cholinesterases
A.M. Nyamsi Hendji,Nicole Jaffrezic-Renault,Claude Martelet,Paul Clechet,A.A. Shlu'ga,V. I. Strikha,L.I. Netchiporuk,Alexey P. Soldatkin,W.B. Wlodarski +8 more
TL;DR: The optimized parameters for the measurement procedure were determined and the detection limits were found to be 10 −12 M for DFP and 10 −6 M for paraoxon-methyl and trichlorfon.
Related Papers (5)
Sensitive detection of pesticides using amperometric sensors based on cobalt phthalocyanine-modified composite electrodes and immobilized cholinesterases
Petr Skládal,Marco Mascini +1 more