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Proceedings ArticleDOI

Cholesterol-enriched erythrocyte-derived optical nanoparticles for NIR fluorescence imaging of intraperitoneal ovarian tumors in mice

16 Mar 2021-Vol. 11660, pp 1166006
TL;DR: Preliminary findings suggest that cholesterol-enrichment of erythrocyte-derived optical nanoparticles can provide a potential capability for enhanced NIR imaging of tumors.
Abstract: We have engineered nanoparticles from erythrocytes doped with the near infrared (NIR) dye, indocyanine green. These particles can be functionalized with various biomolecules such as folate for targeted imaging of cancer cells. Externalization of phosphatidylserine from the inner to the outer leaflet of erythrocyte-derived particles is a common problem during the fabrication process, serving as a signal for phagocytic removal of the particles and reducing tumor targeting. Herein, we demonstrate the effectiveness of membrane cholesterol-enrichment and folate functionalization as a method to increase the efficacy of these particles for NIR fluorescence imaging of intraperitoneal ovarian tumors in mice.
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TL;DR: Progress continues in the development of smaller, more penetrable probes for biological imaging, and the number of probes in this line of research has increased.
Abstract: Progress continues in the development of smaller, more penetrable probes for biological imaging.

3,420 citations

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TL;DR: An overview of the recent surgical intraoperational applications of indocyanine green fluorescence imaging methods, the basics of the technology, and instrumentation used is given.
Abstract: The purpose of this paper is to give an overview of the recent surgical intraoperational applications of indocyanine green fluorescence imaging methods, the basics of the technology, and instrumentation used. Well over 200 papers describing this technique in clinical setting are reviewed. In addition to the surgical applications, other recent medical applications of ICG are briefly examined.

1,000 citations

Journal ArticleDOI
TL;DR: Investigations were carried out to determine whether indocyanine green has properties that may render it suitable for assessing liver function and hepatic blood flow in man.
Abstract: Indocyanine green 1 (ICG) is a tricarbocyanine dye (Figure 1) which has been used in the indicator dilution technique for measuring cardiac output (1, 2). Animal studies (3) and preliminary observations of human subjects (4, 5) have suggested that this dye may have characteristics that could make its uptake, storage, and excretion by the liver helpful indices of hepatic function. The present investigations were carried out to determine whether indocyanine green has properties that may render it suitable for assessing liver function and hepatic blood flow in man.

880 citations

Journal ArticleDOI
TL;DR: New findings from this study show that FRalpha expression is maintained on metastatic foci and recurrent tumors, suggesting that novel folate-targeted therapies may hold promise for the majority of women with either newly diagnosed or recurrent ovarian cancer.

374 citations

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TL;DR: Developments indicate that RBC‐inspired delivery systems will result in next‐generation nanomedicine with extensive medical applications, with an emphasis on how growing understanding of fundamental biological properties of natural RBCs has been applied in the design and engineering of these delivery systems.
Abstract: Herein recent progress in developing red blood cell (RBC)-inspired delivery systems is reviewed, with an emphasis on how our growing understanding of fundamental biological properties of natural RBCs has been applied in the design and engineering of these delivery systems. Specifically, progress achieved in developing carrier RBCs, a class of delivery vehicles engineered by directly loading natural RBCs with therapeutic agents, will be reviewed. Then alternative approaches to engineering synthetic vehicles through mimicking the mechanobiological and chemico-biological properties of natural RBCs will be considered. The synthesis and application of RBC membrane-derived vesicles, of which the natural RBC membranes are collected and directly utilized to prepare drug carriers, will then be discussed. Finally, a recent approach in engineering RBC membrane-camouflaged nanoparticle systems that combine advantages of natural RBCs and synthetic biomaterials will be highlighted. These developments indicate that RBC-inspired delivery systems will result in next-generation nanomedicine with extensive medical applications.

241 citations