Journal ArticleDOI
Cloning and DNA sequence of plasmid determinant iss , coding for increased serum survival and surface exclusion, which has homology with lambda DNA
TLDR
Escherichia coli K12 cells carrying a cloned 1.4 kb HindIII fragment from plasmid ColV2-K94, showed increased survival in guinea pig serum and southern blotting suggested homology with bacteriophage lambda DNA and to the insertion element IS2.Abstract:
Escherichia coli K12 cells carrying a cloned 1.4 kb HindIII fragment from plasmid ColV2-K94, showed increased survival in guinea pig serum. The recombinant plasmid also conferred group II surface exclusion, i.e. the cells were reduced in recipient ability towards the incoming plasmid R538drd in conjugation experiments. Southern blotting suggested homology with bacteriophage lambda DNA and to the insertion element IS2. Determination of the DNA sequence of the fragment demonstrated the presence of a truncated IS2 (165 bp), separated by 250 bp from a 900 bp stretch of homology with lambda DNA, beginning within the Rz gene and continuing in the rightward direction on the lambda map. A 97 amino acid open reading frame (ORF) adjacent to Rz and on the opposite strand, remained intact in iss, with several amino acid changes. The ORF in iss is preceded by sequences resembling prokaryotic ribosome binding sites and promoters.read more
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Extended Virulence Genotypes of Escherichia coli Strains from Patients with Urosepsis in Relation to Phylogeny and Host Compromise
James R. Johnson,Adam L. Stell +1 more
TL;DR: These findings provide novel insights into the VFs of extraintestinal pathogenic E. coli and demonstrate the new PCR assay's utility for molecular epidemiological studies.
Journal ArticleDOI
Comparison of Escherichia coli isolates implicated in human urinary tract infection and avian colibacillosis.
Kylie E. Rodriguez-Siek,Catherine W. Giddings,Curt Doetkott,Timothy J. Johnson,Mohamed K. Fakhr,Lisa K. Nolan +5 more
TL;DR: The potential for APEC to act as human UPEC or as a reservoir of virulence genes for UPEC should be considered, but significant differences in the prevalence of the traits occurred across the two groups, suggesting that if APEC are involved in human urinary tract infections, they are not involved in all of them.
Journal ArticleDOI
Characterizing the APEC pathotype
Kylie E. Rodriguez-Siek,Catherine W. Giddings,Curt Doetkott,Timothy J. Johnson,Lisa K. Nolan +4 more
TL;DR: The majority of the APEC isolates surveyed shared a common set of putative virulence genes, many of which have been localized to an APEC plasmid known as pTJ100, which may prove useful in defining an AP EC pathotype.
Journal ArticleDOI
Avian Colibacillosis and Salmonellosis: A Closer Look at Epidemiology, Pathogenesis, Diagnosis, Control and Public Health Concerns
TL;DR: The vital information on the epidemiology, pathogenesis, diagnosis, control and public health concerns of avian colibacillosis and salmonellosis is provided.
Journal ArticleDOI
A bacterial virulence determinant encoded by lysogenic coliphage λ
Barondess Jj,Jon Beckwith +1 more
TL;DR: The results show that the λ prophage is more tran-scriptionally active than has long been assumed, and suggest that lysogeny may generally have a role in bacterial survival in animal hosts, and perhaps in pathogenesis.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Arapid alkaline extraction procedure forscreening recombinant plasmid DNA
TL;DR: The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.