Journal ArticleDOI
Pedigrees of some mutant strains of Escherichia coli K-12.
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This article is published in Bacteriological Reviews.The article was published on 1972-12-01. It has received 1763 citations till now. The article focuses on the topics: Escherichia coli.read more
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Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
TL;DR: P15A-derived plasmids were not self-transmissible and were mobilized poorly by Hfr strains; however, mobilization was complemented by the presence of a ColE1 plasmid within the same cell.
Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
TL;DR: Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.
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Recalibrated linkage map of Escherichia coli K-12.
B J Bachmann,K B Low,A L Taylor +2 more
TL;DR: This article corrects the article on p. 116 in vol.
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Interposon mutagenesis of soil and water bacteria : a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria
TL;DR: A series of derivatives of the omega interposon that can be used for in vitro insertional mutagenesis are constructed and insertion of these interposons in the plasmid between the promoter and the catechol 2,3-dioxygenase (C23O) gene dramatically reduced the expression of this enzyme in Escherichia coli.
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Bacteriophage lysis: mechanism and regulation.
TL;DR: The available evidence suggests that holins oligomerize to form nonspecific holes and that this hole-forming step is the regulated step in phage lysis, which is as much an essential feature of holin function as is the hole formation itself.
References
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Journal ArticleDOI
Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in escherichia coli K12☆
TL;DR: N-methyl-N′-nitro-N-nitrosoguanidine induces at least one mutation per treated cell under conditions permitting over 50 per cent survival and induced auxotrophs are found.
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On the Regulation of DNA Replication in Bacteria
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Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12.
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Isolation and characterization of recombination-deficient mutants of escherichia coli k12
Alvin J. Clark,Ann Dee Margulies +1 more
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Isolation of an E. coli strain with a mutation affecting DNA polymerase.
Paula De Lucia,John Cairns +1 more
TL;DR: By testing indiscriminately several thousand colonies of mutagenized E. coli, a mutant has been isolated that on extraction proves to have less than I per cent of the normal level of DNA polymerase.