Comparative Evaluation of Eye Irritation Potential of Aqueous Extracts of Sapindus mukorossi, Phyllanthus emblica, Acacia concinna by In vitro and In vivo Methods.

TLDR: Evaluation of eye irritation potential and irritation index of aqueous extracts of fruits of Sapindus mukorossi, Phyllanthus emblica and pods of Acacia concinna by in vitro, Hen€s Egg Chorioallantoic Membrane Test (HET -CAM) and in vivo acute eye irritation test in rabbits.

Abstract: The location of the eye and its anatomy predisposes it to exposure to a variety of environmental conditions and substances on a daily basis and they can be exposed to cosmetic products. Injury from ocular exposure to a variety of chemical agents can lead to a range of adverse effects with the most extreme being blindness. Th e evaluation of eye irritation potential for a cosmetic product and its ingredients is essential to provide reassu rance that a product is safe for consumers. Aim: Present study is to evaluate the eye irritation potential and c ompare irritation index of aqueous extracts of fruits of Sapindus mukorossi, Phyllanthus emblica and pods of Acacia concinna by in vitro, Hen€s Egg Chorioallantoic Membrane Test (HET -CAM) and in vivo acute eye irritation test in rabbits. Materials and Methods: HET  CAM assay uses the CAM of a 10-day-old fertilized hen€s egg. The CAM, a membrane which surrounds the developing chick embryo is highly vascularized extracts of above plants were delivered to the CAM surface and end points were observed are hemorrhage, lysis, coagulation for a perio d of 300 seconds and irr itation index is calculated. Acute eye irritation test is carried out in albino rabbits. 0.1mL of extract applied in conjunctival sac of one eye. Results: The irritation score of S.mukorossi, P.emblica, A.concinna were 8.56, 0.38, 3.29, re spectively. Conclusion: S.mukorossi is categorized under 2B and both P.emblica and A.concinna doesn€t comes under either category 1 or 2 as per the harmonized integrated classification system.