Comparison of counts using a breed-type smear and millipore membrane methods on fresh and preserved milk samples.
C.L. Duitschaever,G.C. Ashton +1 more
TLDR
The millipore membrane count is a better method for determining somatic cells; significantly lower bacterial counts were obtained with it than the direct microscopic count; and the preservative had a marked adverse effect upon the stainability of bacteria independent of the method.About:
This article is published in Journal of Dairy Science.The article was published on 1968-05-01 and is currently open access. It has received 6 citations till now.read more
Citations
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Journal ArticleDOI
Semi-automated counting of bacteria and somatic cells in milk using epifluorescence microscopy and television image analysis.
TL;DR: The semi-automated DEFT count of bacteria and somatic cells correlated well with the plate count and Coulter count respectively, with correlation coefficients of 0.83 and 0.81.
Journal Article
The diagnosis of bovine mastitis with particular reference to subclinical mastitis : a critical review of relevant literature
TL;DR: The reliability of the current diagnosis of mastitis is investigated, as well as the significance of epithelial cells in milk and the cytological significance of leucocytes in milk, in the context of modern mastitis.
Journal Article
The effects of storage and method of fixation on somatic cell counts in bovine milk
I R Dohoo,I McMillan,A H Meek +2 more
TL;DR: In fresh milk samples and potassium dichromate preserved milk samples the use of glutaraldehyde as a fixative required adjustment of the threshold setting on the cell counter in order to produce results comparable to those obtained from formalin fixed samples.
Book ChapterDOI
Development and evaluation of membrane filtration techniques in microbial analysis
TL;DR: Membrane filters were used before agar was adopted by microbiologists, when Fick (1855) made nitrocellulose membranes as substitutes for animal membranes in dialysis experiments, but it was some time before a useful preparation method was developed.
Journal ArticleDOI
Nuclepore Membrane Filters for Milk Somatic Cell Estimations in Low Count Milk
TL;DR: The method appears suitable for application to low count milk from normal quarters and precision and accuracy were compared with the direct microscopic somatic cell count modified for low cell count milk.
References
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Journal ArticleDOI
Rapid Graphical Method for Estimating the Precision of Direct Microscopic Counting Data: I. Preliminary Evaluation of Technique
TL;DR: A graphical method of estimating the 90, 95, or 99% confidence intervals of direct microscopic counting data is presented, based on the assumption that the normal distribution approximates the Poisson distribution.
Journal ArticleDOI
Technique for microscopic count of microorganisms directly on membrane filters.
TL;DR: In an attempt to apply the direct microscopic method to the counting of samples with low bacterial populations, various concentration techniques were employed, including sedimentation, centrifugation, vacuum distillation, and filtration.
Journal ArticleDOI
Cells in bovine milk: differential staining in suspension: collecting, counting and examining on millipore membrane.
C. L. Duitschaever,A. G. Leggatt +1 more
TL;DR: Differential staining of somatic cells in bovine milk preserved with 12 mg of HgCl2 and 52 mg of K2Cr2O7 in 50 ml was performed as follows.
Journal ArticleDOI
Visibilizing microorganisms on membrane filter surface.
Oscar W. Richards,Wilfred Krabek +1 more
TL;DR: It is suggested that viruses may produce changes in tumor tissue cultures other than oncolysis, which may prove of significance in determining their cytopathogenic effect.