Concentration of poliovirus from tap water using positively charged microporous filters
Mark D. Sobsey,B L Jones +1 more
TLDR
Electropositive filters appear to offer distinct advantages over conventional negatively charged filters for concentrating enteric viruses from water, and their behavior tends to confirm the importance of electrostatic forces in virus recovery from water by microporous filter adsorption-elution methods.Abstract:
Microporous filters that are more electropositive than the negatively charged filters currently used for virus concentrations from water by filter adsorption-elution methods were evaluated for poliovirus recovery from tap water. Zeta Plus filters composed of diatomaceous earth-cellulose-"charge-modified" resin mixtures and having a net positive charge of up to pH 5 to 6 efficiently adsorbed poliovirus from tap water at ambient pH levels 7.0 to 7.5 without added multivalent cation salts. The adsorbed virus were eluted with glycine-NaOH, pH 9.5 to 11.5. Electropositive asbestos-cellulose filters efficiently adsorbed poliovirus from tap water without added multivalent cation salts between pH 3.5 and 9.0, and the absorbed viruses could be eluted with 3% beef extract, pH 9, but not with pH 9.5 to 11.5 glycine-NaOH. Under water quality conditions in which poliovirus recoveries from large volumes of water were less than 5% with conventional negatively charged filters and standard methods, recoveries with Zeta Plus filters averaged 64 and 22.5% for one- and two-stage concentration procedures, respectively. Electropositive filters appear to offer distinct advantages over conventional negatively charged filters for concentrating enteric viruses from water, and their behavior tends to confirm the importance of electrostatic forces in virus recovery from water by microporous filter adsorption-elution methods.read more
Citations
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Book ChapterDOI
Applied and Theoretical Aspects of Virus Adsorption to Surfaces
TL;DR: This chapter highlights how the current understanding of the mechanisms and factors influencing virus adsorption can be used to interpret and control virus behavior in the environment.
Journal ArticleDOI
Development of a Virus Concentration Method and Its Application to Detection of Enterovirus and Norwalk Virus from Coastal Seawater
TL;DR: This method showed higher recovery yields than that of the conventional method using positively charged membrane when applied to seawater and is also free from beef extract elution, which has an inhibitory effect in the subsequent viral genome detection by reverse transcription-PCR.
Journal ArticleDOI
Viral Pollution in the Environment and in Shellfish: Human Adenovirus Detection by PCR as an Index of Human Viruses
TL;DR: The results suggest that the detection of adenoviruses by PCR could be used as an index of the presence of human viruses in the environment where a molecular index is acceptable.
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Isoelectric points of viruses
Benjamin Michen,Thomas Graule +1 more
TL;DR: The IEP measurements of viruses that replicate in hosts of kingdom plantae, bacteria and animalia are reviewed and a discrepancy is discussed and should be considered when IEP values are used to account for virus sorption processes.
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Outbreak of viral gastroenteritis due to a contaminated well. International consequences.
Michael Beller,Andrea Ellis,Spencer H. S. Lee,Michael A. Drebot,Sue Anne Jenkerson,Elizabeth Funk,Mark D. Sobsey,Otto D. Simmons,Stephan S. Monroe,Tamie Ando,Jacqueline S. Noel,Martin Petric,John P. Middaugh,John S. Spika +13 more
TL;DR: The investigation documented SRSVs in a vehicle epidemiologically linked to a gastroenteritis outbreak, demonstrating the power of molecular detection and identification and underscore the importance of fundamental public health practices such as restaurant inspection, assurance of a safe water supply, and disease surveillance.
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