Conjugative transfer of the transposon Tn919 to lactic acid bacteria
TLDR
Analysis of S. lactis MG1363 Smr Tcr transconjugants showed that insertion of Tn919 into the chromosome was site-specific, and it was concluded that streptococcal transposon Tn 919 was transferred from Streptococcus faecalis GF590 to selected Group N StrePTococcus strains and to one strain each.Abstract:
The streptococcal transposon Tn919 was transferred from Streptococcus faecalis GF590 to selected Group N Streptococcus strains and to one strain each of Lactobacillus plantarum and Leuconostoc cremoris, using the filter mating method. An S. lactis MG1363 Rifr Tcr transconjugant also acted as a donor, but was less efficient than GF590. Frequencies of transfer varied between 4.0 × 10−8 and 5.29 × 10−5 per recipient. Further analysis of S. lactis MG1363 Smr Tcr transconjugants showed that insertion of Tn919 into the chromosome was site-specific.read more
Citations
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Journal ArticleDOI
Gene cloning and expression in lactic streptococci
TL;DR: Several host vector systems have been described which currently are used in the cloning and expression of homologous and heterologous genes. as discussed by the authors described the essential elements of these systems, various cloning strategies and the first successful cloning experiments with emphasis on the molecular organization of proteinase genes.
Journal ArticleDOI
Molecular cloning and nucleotide sequence of the gene encoding the major peptidoglycan hydrolase of Lactococcus lactis, a muramidase needed for cell separation.
Girbe Buist,Jan Kok,Kees Leenhouts,Magdalena Dabrowska,Gerhardus Venema,Alfred J. Haandrikman +5 more
TL;DR: A gene of Lactococcus lactis subsp.
Journal ArticleDOI
Characterization of the novel nisin-sucrose conjugative transposon Tn5276 and its insertion in Lactococcus lactis.
P. J. G. Rauch,W.M. de Vos +1 more
TL;DR: A novel, chromosomally located conjugative transposon in Lactococcus lactis, Tn5276, was identified and characterized and encodes the production of and immunity to nisin, a lanthionine-containing peptide with antimicrobial activity, and the capacity to utilize sucrose via a phosphotransferase system.
Book ChapterDOI
The genus Lactococcus
TL;DR: The first bacterial pure culture on earth, obtained and scientifically described by Joseph Lister (1873) was Lactococcus lactis, at that time called: ‘Bacterium lactis’ as discussed by the authors.
Journal ArticleDOI
Vancomycin-resistant Leuconostoc mesenteroides and Lactobacillus casei synthesize cytoplasmic peptidoglycan precursors that terminate in lactate.
TL;DR: To determine whether the mechanisms of resistance are similar in intrinsically resistant bacteria, cytoplasmic peptidoglycan precursors were isolated from Leuconostoc mesenteroides and Lactobacillus casei and analyzed by mass spectrometry, revealing structures consistent with UDP-N-acetylmuramyl-L-Ala-D-Glu-L
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