Construction of an unstable Ring-X chromosome bearing the autosomal dopa decarboxylase gene in Drosophila melanogaster and analysis of Ddc mosaics
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TLDR
An unstable Ring-X chromosome, DDC+ -Ring-X carrying a cloned Dopa decarboxylase (Ddc) encoding segment was constructed andalyses of Ddc mosaics revealed that while there was no absolute requirement for the Ddc+ expression in either the epidermis or the nervous system, very large mutant clones did affect the viability of the mosaic.Abstract:
An unstable Ring-X chromosome, Ddc+ -Ring-X carrying a cloned Dopa decarboxylase (Ddc) encoding segment was constructed. The construction involved a double recombination event between the unstable Ring-X, R(1)wvC and a Rod-X chromosome which contained a P-element mediated Ddc+ insert. The resulting Ddc+ -Ring-X chromosome behaves similarly to the parent chromosome with respect to somatic instability. The Ddc+ -Ring-X chromosome was used to generate Ddc mosaics. Analyses of Ddc mosaics revealed that while there was no absolute requirement for the Ddc+ expression in either the epidermis or the nervous system, very large mutant clones did affect the viability of the mosaic.read more
Citations
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Book ChapterDOI
The Genetics Of Biogenic Amine Metabolism, Sclerotization, And Melanization In Drosophila Melanogaster*
TL;DR: The availability of mutations that interrupt biogenic-amine metabolism in an experimentally suitable organism such as Drosophila should provide powerful analytical tools for neurochemists, neurophysiologists, and behavioral biologists.
Journal ArticleDOI
Serotonin-containing neurons in Drosophila melanogaster: development and distribution
Ana Maria Vallés,Kalpana White +1 more
TL;DR: Antibodies made against serotonin (5HT) were used to identify the serotonin neuronal system in the developing and adult nervous system of Drosophila melanogaster and comparison of the 5HT‐IR pattern within different insect species is presented.
Journal ArticleDOI
Catecholamine‐containing neurons in Drosophila melanogaster: Distribution and development
Vivian Budnik,Kalpana White +1 more
TL;DR: It is suggested that these larval CA neurons are maintained throughout the ontogeny of the fly and that the adult CA pattern is composed of embryonic neurons and neurons that differentiate during metamorphosis.
Book ChapterDOI
Molecular and genetic approaches to neurotransmitter and neuromodulator systems in Drosophila.
Linda L. Restifo,Kalpana White +1 more
TL;DR: This chapter summarizes studies of compounds that are postulated to mediate or modulate neurotransmission in Drosophila with an emphasis on the genetic approaches used to study them.
Journal ArticleDOI
Genetic dissection of dopamine and serotonin synthesis in the nervous system of Drosophila melanogaster
Vivian Budnik,Kalpana White +1 more
TL;DR: It is shown that normal characteristics of catecholamine neurons, such as the presence of the enzyme DOPA decarboxylase and the selective uptake properties are normal in pale mutants, and suggests that pale is either the tyrosine hydroxylase structural gene, or a gene controlling tyrosin hydroxyase activity in Drosophila.
References
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Journal ArticleDOI
Genes and Developmental Pathways
TL;DR: An approach is discussed which makes use of a series of pseudoallelic genes in Drosophila which have, in several instances, been profitably exploited in the bacteria to learn more about how genes control biochemical pathways.
Journal ArticleDOI
Genetic Dissection of Monoamine Neurotransmitter Synthesis in Drosophila
TL;DR: It is suggested that there are two different aromatic amino acid decarboxylases in Drosophila brains, one that decar boxylates L-dopa and 5-hydroxytryptophan and another that decARboxylates tyrosine.
Journal ArticleDOI
Acetylcholinesterase mutants in drosophila and their effects on the structure and function of the cental nervous system
TL;DR: Mutations that eliminate acetylcholinesterase (AChE) activity were studied in Drosophila melanogaster to study the effects of disrupted ACholine metabolism on the form and function of the central nervous system as mentioned in this paper.
Journal ArticleDOI
Neuropeptide‐Fmrfamide‐Like immunoreactivity in drosophila: Development and distribution
TL;DR: Localization of immunoreactivity in the central nervous system of early larval stage revealed that the majority of the prominent FMRFamide‐like immunoreactive neurons were already differentiated.
Journal ArticleDOI
Mutations in the dopa decarboxylase gene affect learning in Drosophila.
TL;DR: Moderate Ddc mutations affected the acquisition of learned responses while leaving memory retention unaltered, in contrast to the mutations dunce, rutabaga, and amnesiac , which primarily affect short-term memory.
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