Continuous-Flow Ultracentrifugation of Canine Distemper Virus and Infectious Canine Hepatitis Virus
John A. Elliott,Wayne L. Ryan +1 more
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The use of a Spinco L-4 zonal centrifuge with the B-XVI continuous-flow rotor for the purification of canine distemper and infectious canine hepatitis viruses is described and the purity of the virus was increased by 99%.Abstract:
The use of a Spinco L-4 zonal centrifuge with the B-XVI continuous-flow rotor for the purification of canine distemper and infectious canine hepatitis viruses is described. Up to 68 liters of virus was processed at one time. Infectious canine hepatitis virus was found to band at 39% sucrose and canine distemper virus banded between 32 and 48% sucrose. The virus was concentrated 10-fold, and the purity of the virus, as measured by protein concentration, was increased by 99%.read more
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Canine distemper virus
M. J. G. Appel,J. H. Gillespie +1 more
TL;DR: Canine distemper (CD) is the only virus disease in dogs with a high mortality rate, and it has a worldwide distribution and only few dogs in isolated areas remain without contact with the virus.
Journal ArticleDOI
Preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow ultracentrifugation.
Doria R. Dubois,Doria R. Dubois,Ernest C. Cutchins,Ernest C. Cutchins,Sanford Berman,Sanford Berman,Joseph P. Lowenthal,Joseph P. Lowenthal,Robert L. Timchak,Robert L. Timchak +9 more
TL;DR: Based on chemical assays, the Genetron zonal rickettsial suspensions were found to be as pure as the ricketsial suspensions which were prepared by the ether extraction method currently in use for producing Q fever vaccines for human use.
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Continuous-flow ultracentrifugation in preparative biochemistry.
TL;DR: It appears that the Oak Ridge derived continuous-flow rotors have filled an important niche in the biochemical process industries for the large-scale purification of viruses, particularly those used in vaccines.
Journal ArticleDOI
Buoyant density of canine distemper virus.
L. A. Phillips,R. H. Bussell +1 more
TL;DR: The buoyant densities for both the virion and CF antigens of CDV were in close agreement with those previously reported for measles virus.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Purification of Large Quantities of Influenza Virus by Density Gradient Centrifugation
TL;DR: The combined process: absorption and elution of influenza virus with barium sulfate followed by concentration and isopycnic banding of the virus in a buffered sucrose gradient is studied.
Journal ArticleDOI
Use of Zonal Ultracentrifuge Systems for Biophysical Studies of Rhinoviruses
TL;DR: This paper reports the use of zonal ultracentrifuge techniques to conduct biophysical studies of rhinoviruses grown with WI-38 cells and finds two particle sizes found to be associated with the virus-infected cell harvests.
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