Journal ArticleDOI
Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers
David K. Gardner,Pauline Vella,Michelle Lane,L Wagley,Terry Schlenker,William B. Schoolcraft +5 more
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TLDR
Viable human blastocysts can be obtained in sequential culture media in the absence of coculture and serum and will facilitate high PRs while limiting the number of embryos transferred and therefore minimizes the risk of multiple gestation.About:
This article is published in Fertility and Sterility.The article was published on 1997-01-01. It has received 604 citations till now. The article focuses on the topics: Blastocyst Transfer & Embryo transfer.read more
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Births: final data for 2002.
Joyce A Martin,Brady E Hamilton,Paul D Sutton,Stephanie J. Ventura,Fay Menacker,Martha L Munson +5 more
TL;DR: The cesarean delivery rate climbed to the highest level ever reported in the United States (26.1 percent) and the rate of vaginal birth after previous cedarean plummeted 23 percent to 12.6 percent for 2002.
Journal ArticleDOI
Embryonic stem cells.
Helen J. Rippon,Anne E. Bishop +1 more
TL;DR: This work reviews the history of murine and human ES cell Lines, including practical and ethical aspects of ES cell isolation from pre‐implantation embryos, maintenance of undifferentiated ES cell lines in the cell culture environment, and differentiation of ES cells in vitro and in vivo into mature somatic cell types.
Journal ArticleDOI
A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization.
TL;DR: The ability to transfer just two blastocysts while maintaining high pregnancy rates will help to eliminate high order multiple gestations and improve the overall efficiency of human IVF.
Births: final data for 2004.
Joyce A Martin,Brady E Hamilton,Paul D Sutton,Stephanie J. Ventura,Fay Menacker,Sharon Kirmeyer +5 more
TL;DR: The cesarean delivery rate jumped 6 percent to another all-time high, whereas the rate of vaginal birth after previous cesAREan fell by 13 percent, and preterm and low birthweight rates continued their steady rise.
References
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Journal ArticleDOI
An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro
TL;DR: The in-vitro development of 1-cell embryos beyond the 2-cell stage in response to the removal of glucose and the addition of glutamine to the culture medium suggests that glucose may block some essential metabolic process, and that glutamine may be a preferred energy substrate during early development for these mouse embryos.
Journal ArticleDOI
Culture of preimplantation embryos: facts and artifacts
TL;DR: In this review, comparative information on epigenetic regulation of embryo development is discussed, including information on energy substrate and amino acid preferences of embryos and improvements in the design of culture media are discussed, so that higher yields and increased viability of embryos are achieved.
Journal ArticleDOI
Culture and selection of viable blastocysts: a feasible proposition for human IVF?
David K. Gardner,Michelle Lane +1 more
TL;DR: The development of suitable non-invasive tests of embryo viability should further increase the overall success of human IVF by the ability to select before transfer those blastocysts most able to establish a pregnancy.
Journal ArticleDOI
Differential regulation of mouse embryo development and viability by amino acids
Michelle Lane,David K. Gardner +1 more
TL;DR: The highest rates of development in vitro and viability after transfer were achieved when embryos were cultured with non-essential amino acids and glutamine to the eight-cell stage followed by development to the blastocyst stage in the presence of all 20 amino acids.
Book
Oxford reviews of reproductive biology
TL;DR: In this article, the biology of the placental prolactin growth hormone gene family and the development of the normal human breast was discussed. And the physiology of penile erection was investigated in domestic ruminants.
Related Papers (5)
Culture and selection of viable blastocysts: a feasible proposition for human IVF?
David K. Gardner,Michelle Lane +1 more