Journal ArticleDOI
Cyclic AMP and adenyl cyclase in the developing rat brain.
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The level of endogenous cyclic AMP in the rat brain in vivo began to increase markedly between the third and sixth days after birth, as did the ability of norepinephrine to stimulate the formation of cyclicAMP in brain tissue in vitro.Abstract:
The level of endogenous cyclic AMP in the rat brain in vivo began to increase markedly between the third and sixth days after birth, as did the ability of norepinephrine to stimulate the formation of cyclic AMP in brain tissue in vitro. Adenyl cyclase activity in broken cell preparations, when measure in the absence of sodium fluoride, increased with age up to a point, but began to decline between the fifth and ninth days postpartum. Activity continued to increase when measured in the presence of fluoride, suggesting that the apparent stimulatory effect of this ion may in fact be the reversal of an inhibitory influence which is absent or almost absent at birth. Cyclase activity at all ages was restricted to particulate matter, whereas apparent phosphodiesterase activity was present in particulate as well as soluble fractions. The catabolic system for cyclic AMP developed in a similar manner in both fractions. Theophylline produced the same degree of inhibition of this system at all ages.read more
Citations
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Journal ArticleDOI
Radioimmunoassay for Cyclic Nucleotides I. PREPARATION OF ANTIBODIES AND IODINATED CYCLIC NUCLEOTIDES
TL;DR: Sensitive and reproducible radioimmunoassays have been developed for cyclic adenosine 3',5'-monophosphate (cAMP), cyclic guanosine 3,5'- monophosphates (cGMP), cycled inosine 3,'5'-MonophosphATE (cIMP), and cyclic uridine 3',4'-monophile (cUMP) based upon competition of the cyclic nucleotide with isotopically labeled cyclic
Journal ArticleDOI
Chemical messengers in development: a hypothesis.
TL;DR: The hypothesis presented here suggests that simple derivatives of metabolites, including neurotransmitters and cyclic nucleotides, are linked together as regulatory molecules throughout the eukaryotes and are suggested to have a more general role in information transmission in eucaryotes than is generally accepted.
Book ChapterDOI
The role of cyclic nucleotides in central synaptic function.
TL;DR: Sutherland et al. as mentioned in this paper showed that cyclic AMP functioned as an intracellular second messenger, synthesized in response to certain hormones and which, by activating the appropriate sequence of enzymes, produced the specific biologic response of the target cell to the hormone.
Journal ArticleDOI
Radioimmunoassay for cyclic nucleotides. 3. Effect of ischemia, changes during development and regional distribution of adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate in mouse brain.
TL;DR: The changes in cGMP levels between 7 days and adulthood were quite unlike those of cAMP, and there was no change in forebrain c GMP, brain stem cG MP increased 2-fold, and in cerebellum cGmp rose dramatically, to a level 13 times that at 7 days.
References
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Journal ArticleDOI
Some Aspects of the Biological Role of Adenosine 3',5'-monophosphate (Cyclic AMP)
TL;DR: The evidence presented supports the hypothesis that the positive inotropic response to the catecholamines is mediated by cyclic AMP.
Journal ArticleDOI
Adenyl Cyclase I. DISTRIBUTION, PREPARATION, AND PROPERTIES
Journal ArticleDOI
Adenosine 3',5'-monophosphate in biological materials. II. The measurement of adenosine 3',5'-monophosphate in tissues and the role of the cyclic nucleotide in the lipolytic response of fat to epinephrine.
Journal ArticleDOI
A radioisotopic method for measuring the formation of adenosine 3',5'-cyclic monophosphate in incubated slices of brain.
TL;DR: The method was used to study the effect of histamine, norepinephrine, andAdenosine on the accumulation of adenosine 3′,5′‐cyclic monophosphate in incubated slices of brain.
Journal ArticleDOI
Adenyl Cyclase in Fat Cells I. PROPERTIES AND THE EFFECTS OF ADRENOCORTICOTROPIN AND FLUORIDE
TL;DR: It was concluded that ACTH and fluoride ion activate the same enzyme and alter the same kinetic parameter involved in activation, but do so by different means.