Journal ArticleDOI
Cytodifferentiation in the Rana pipiens oocyte. I. Association between mitochondria and nucleolus-like bodies in young ootes.
TLDR
Small oocytes in adult Rana pipiens contain a number of cytoplasmic bodies which resemble nucleoli in size, shape, and staining reactions, but these nucleolus-like bodies are, however, more finely granular than nucleoli.About:
This article is published in Journal of Ultrastructure Research.The article was published on 1969-07-01. It has received 80 citations till now. The article focuses on the topics: Nucleolus.read more
Citations
More filters
Book ChapterDOI
Germ plasm and the differentiation of the germ cell line.
TL;DR: This chapter discusses germ plasm and the differentiation of the germ cell line and the histochemical evidence for the presence of RNA in polar granules of insects and germinal plasm of amphibians indicates that RNA is present only in eggs and early embryos, while a protein component is present throughout the germ cycle.
Book ChapterDOI
The problem of germ cell determinants.
H. W. Beams,Richard G. Kessel +1 more
TL;DR: Molecular aspects of chromatin elimination in Ascaris lumbricoides have recently been investigated specifically with respect to a comparison of the informational content of AsCaris cells before and after chromatin eliminated, and studies indicate that chromatin removal leads to a quantitative difference in information content of the somatic cell line versus the germ cell line.
Book ChapterDOI
Annulate Lamellae: A Last Frontier in Cellular Organelles
TL;DR: In this paper, the authors highlight annulate lamellae (AL) that are porous and often stacked parallel membranes that are largely confined to the cytoplasm of cells possessing them, but in a few cell types they may also be located inside the nucleus.
Journal ArticleDOI
Outet mitochondrial membrane continuous with endoplasmic reticulum
TL;DR: Continuity of outer mitochondrial membrane with tubular profiles of endoplasmic reticulum (ER) character is found in rat liver hepatocytes and in the ciliate Tetrahymena pyriformis, and it is hypothesized that this cisternal continuity provides a route for transfer of special proteins from the rough ER into the mitochondria.
Journal ArticleDOI
An ultrastructural study of primordial germ cells, oogonia and early oocytes in Xenopus laevis.
TL;DR: Electron-microscope observations on the differentiation of germ cells in Xenopus laevis have revealed that the Balbiani body, cytoplasmic nucleolus-like bodies and groups of mitochondria associated with granular material previously reported only in older amphibian oocytes, are also present in the primordial germ cells, oogonia and early meiotic (prediplotene) oocytes of this species.
References
More filters
Journal ArticleDOI
THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY
TL;DR: The stain reported here differs from previous alkaline lead stains in that the chelating agent, citrate, is in sufficient excess to sequester all lead present, and is less likely to contaminate sections.
Journal ArticleDOI
Improvements in epoxy resin embedding methods.
TL;DR: Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenientembedding methods for electron microscopy.
Journal ArticleDOI
Staining of Tissue Sections for Electron Microscopy with Heavy Metals
TL;DR: Certain hitherto unobserved details are revealed and some sort of specificity exists, although the factors involved are not yet understood.
Journal ArticleDOI
Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.
TL;DR: A postfixation in osmium tetroxide, even after long periods of storage, developed an image that—notable in the case of glutaraldehyde—was largely indistinguishable from that of tissues fixed under optimal conditions with osmia tetroxides alone.
Journal ArticleDOI
Embedding in epoxy resins for ultrathin sectioning in electron microscopy.
TL;DR: More rapid than previous techniques, this method gives blocks which do not fracture unduly on trimming and provides sections of soft tissues at 1 μ for phase contrast microscopy, as well as ultrathin sections which cut as easily with glass knives as sections of methacrylate.