Detection of Porphyromonas gingivalis from Periodontal Pocket Infections by Microbial Cultivation and PCR Techniques
TLDR
The study revealed that there are effects of age and sex on isolation rate and the results indicated that percentage of P.gingivalis was detected in 20-30 years old and males were more infected than females.Abstract:
The study aimed to detect P. gingivalis from 49 patients with periodontitis at different ages and both sexes, after determination of pocket depth, types of infection whether chronic or progressive by dentists. Routine culture method was done using selective media and anaerobic condition and compared with species specific polymerase chain reaction (PCR) technique. DNA was extracted from samples and its concentration and purity were determined. The results showed domination of chronic infections and the pocket depths ranged between 3-9mm, as well as the results revealed that isolation percent of P.gingivalis by PCR was more higher than culture method, it was 65.3% and 28.5% respectively. The results also showed that phenol-chloroform was the efficient method for DNA extraction comparing with other methods. The study revealed that there are effects of age and sex on isolation rate and the results indicated that percentage of P.gingivalis was detected in 20-30 years old and males were more infected than females.read more
Citations
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Systemic Immunological Responses Among Dental Infection Patients
TL;DR: The present study conclude that the bacteria isolated from dental infection patients were mixed more than single infection, there were non-significant elevation in IgA, IL-4, and CD8 in patients while IL-7 and CD4 was lower in patients group than in control group, while CD4\CD8 ratio were lower in Patients group, these result reflect the fact that mucosal antigen induce systemic tolerance to some extent.
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Isolation and identification of bacterial species associated with odontogenic infections
TL;DR: The present results approve the existence of mixed bacterial infection with the predominance of Streptococcusviridans, which showed very high susceptibility of isolates to amoxicillin followed by cefotaxime and imipenem.
References
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Development of a Classification System for Periodontal Diseases and Conditions
TL;DR: How the new classification for periodontal diseases and conditions presented in this volume differs from the classification system developed at the 1989 World Workshop in Clinical Periodontics is summarized.
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New Bacterial Species Associated with Chronic Periodontitis
Purnima S. Kumar,Ann L. Griffen,J.A. Barton,Bruce J. Paster,Melvin L. Moeschberger,Eugene J. Leys +5 more
TL;DR: In this article, the purpose of the present investigation was to identify potential periodontal pathogens among newly identified species and phylotypes, and species-specific ribosomal 16S primers for PCR amplification were developed for detection of new species.
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Identification of Candidate Periodontal Pathogens and Beneficial Species by Quantitative 16S Clonal Analysis
TL;DR: Chronic periodontitis is the result of a global perturbation of the oral bacterial ecology rather than a disease-site specific microbial shift, and more differences were found in the bacterial profile between subjects withperiodontitis and healthy subjects than between deep and shallow sites within the same subject.
Journal Article
Development of a classification system for periodontal diseases and conditions.
TL;DR: A revised classification system for periodontal diseases and conditions was developed at the 1999 World Workshop in Clinical Periodontics as discussed by the authors, which was used by the American Academy of Periodontology to organize an international workshop to revise the classification system.
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Porphyromonas gingivalis-epithelial cell interactions in periodontitis
TL;DR: Current knowledge on the molecular cross-talk between P. gingivalis andGingival epithelial cells in the development of periodontitis is reviewed.