Journal ArticleDOI
Detection of potato leafroll virus in single aphids by the reverse transcription polymerase chain reaction and its potential epidemiological application.
TLDR
A reverse transcription and polymerase chain reaction (RT-PCR) system was developed using two 20-mer primers located in the potato leafroll virus (PLRV) capsid gene and a 336-bp PCR product was detected from aphids which had been fed on PLRV-infected plants.About:
This article is published in Journal of Virological Methods.The article was published on 1995-09-01. It has received 85 citations till now. The article focuses on the topics: Potato leafroll virus & Myzus persicae.read more
Citations
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Journal ArticleDOI
Aphid-transmitted potato viruses: The importance of understanding vector biology
TL;DR: This review addresses some of the biological complexities presented by aphid-transmitted viruses of potato, Solanum tuberosum L., and their vectors and discusses the application of this knowledge to the management of potato viruses with particular emphasis on seed potato production.
Journal ArticleDOI
A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves, and tubers.
Xianzhou Nie,Rudra P. Singh +1 more
TL;DR: Comparison of RP primed cDNAs from dormant or sprouted tubers and leaves showed that for some cultivars, such as 'Shepody', leaves were more reliable for PVY and PLRV detection than the tubers, in both the d- and m-RT-PCR.
Journal ArticleDOI
Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA
Antonio Olmos,M. A. Cambra,M. A. Dasi,Thierry Candresse,Olga Esteban,María Teresa Gorris,M. Asensio +6 more
TL;DR: Testing of 228 PPV-infected fruit tree samples indicated that so far only PPV type D appears to be present in Spain and in Chile, and increased sensitivity provided by heminested-PCR allowed the detection of PPV targets of D and M types, in wingless individuals of the aphid vector Aphis gossypii.
Book ChapterDOI
Epidemiology and Field Control of PVY and PLRV
TL;DR: Hane and Hamm as discussed by the authors showed that primary infection with potato leafroll virus (PLRV) can affect potato quality by causing phloem net necrosis, a darkening of the vascular bundle that becomes more pronounced during storage.
Journal ArticleDOI
Detection of stylet-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction.
TL;DR: Significant difference in virus detection was observed whether the aphids were tested immediately after exposure to virus sources or stored for up to 45 days in ethanol at room temperature.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Compendium of potato diseases.
TL;DR: The following are some examples of potato diseases that have been recorded in the last century:
Journal ArticleDOI
Potato leafroll virus
Dick Peters,R.C.F Jones +1 more
TL;DR: A datasheet on potato leafroll virus covers Identity, Overview, Distribution, Dispersal, Hosts/Species Affected, Vectors & Intermediate Hosts, Diagnosis, Biology & Ecology, Seedborne Aspects, Impacts, Prevention/Control, Further Information as discussed by the authors .
Related Papers (5)
Detection of stylet-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction.
A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves, and tubers.
Xianzhou Nie,Rudra P. Singh +1 more
Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.
M. F. Clark,A. N. Adams +1 more