Patent
Determination of sperm concentration and viability for artificial insemination
TLDR
In this article, a method for simultaneous determination of the total concentration of sperm cells and the proportion of live sperm cells in a semen sample is provided, which can distinguish between live, dead and/or dying sperm cells by detection of the fluorescent response from the sperm cells.Abstract:
A method for the simultaneous determination of the total concentration of sperm cells and the proportion of live sperm cells in a semen sample is provided. By selective staining of live, dead and/or dying sperm cells, for example using one or more fluorochromes, the detection means can distinguish between live, dead and/or dying sperm cells, for example by detection of the fluorescent response from the sperm cells. The selective staining may be performed at room temperature and in concentrations below standard concentrations. The determination may be performed by selective counting of cells of each fluorescent quality for example by using a flow cytometer having internal concentration standard means. Furthermore, the likelihood of fertilizing a female animal with an insemination dose, taken from a semen sample having a known total concentration of sperm cells and a known proportion of live sperm cells, is predicted on the basis of the thus determined total concentration of sperm cells and the proportion of live sperm cells.read more
Citations
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Patent
Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
Gary Durack,Jeffrey D. Wallace,Gary P. Vandre,Lon A. Westfall,Jeremy T. Hatcher,Niraj V. Nayak +5 more
TL;DR: In this article, a multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of radiation and a beam splitter for producing multiple beams of radiation for each interrogation location associated with each flow channel of the system.
Patent
System for analyzing particles using multiple flow cytometry units
Gary Durack,Jeffrey D. Wallace,Gary P. Vandre,Lon A. Westfall,Jeremy T. Hatcher,Niraj V. Nayak +5 more
TL;DR: In this article, a multichannel system for classifying particles according to one or more characteristics of the particles includes a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing particles using a beam of electromagnetic radiation.
Patent
Method of cryopreserving selected sperm cells
TL;DR: In this paper, a method of cryopreserving sperm that have been selected for a specific characteristic was proposed, such as sex-type, and used for artificial insemination or in vitro fertilization.
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High resolution flow cytometer
TL;DR: In this paper, the authors present a high resolution particle differentiation process and separation system that provides enhanced resolution of particles based upon selected particle characteristics, including an enhanced resolution flow cytometer.
Patent
System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
TL;DR: In this paper, the authors describe methods for handling, separating, packaging, and utilization of spermatozoa that can be derived from previously frozen sperm sampels collected forma male mammel.
References
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Journal ArticleDOI
Viability assessment of mammalian sperm using SYBR-14 and propidium iodide.
D. L. Garner,Larry Johnson +1 more
TL;DR: The proportion of living sperm in semen from six representative mammals was assessed by means of a dual staining technique using the stains SYBR-14 and propidium iodide (PI), a newly developed fluorescent nucleic acid stain that stained the nuclei ofliving sperm bright green as determined by simultaneous examination of fluorescence and motility.
Journal ArticleDOI
Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa.
TL;DR: The ability of JC-1 to discriminate between mitochondria exhibiting high membrane potential from those having low to medium membrane potential provided a more rigorous estimate of metabolic function than the other two fluorescent stains.
Journal ArticleDOI
Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide.
TL;DR: The combination of SYBR-14 and PI was determined to be an effective tool for assessing the viability of fresh or cryopreserved sperm.
Journal ArticleDOI
Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility.
TL;DR: This is the first report of a sperm “motility” assay based on measurements of mitochondriab membrane potential, and offers an advantage over the slower, semiquantitative methods used currently for analyses of sperm motility and viability.
Journal ArticleDOI
Chlortetracycline analysis of boar spermatozoa after incubation, flow cytometric sorting, cooling, or cryopreservation.
W.M.C. Maxwell,Larry Johnson +1 more
TL;DR: The hypothesis that incubation, flow cytometric sorting, cooling, and cryopreservation cause changes to boar sperm membranes which resemble capacitation and the acrosome reaction was tested.