Development of two-cell mouse embryos in the absence of a fixed-nitrogen source
Judith A. Cholewa,W. K. Whitten +1 more
TLDR
Mouse embryos developed from the eight-cell stage to expanded blastocysts in a defined medium in which glycine was the only source of fixed nitrogen, confirming findings confirmed by Bunim (1960), who also showed that glycine could be replaced by Versene.Abstract:
Mouse embryos developed from the eight-cell stage to expanded blastocysts in a defined medium in which glycine was the only source of fixed nitrogen (Whitten, 1957). These findings were confirmed by Bunim (1960), who also showed that glycine could be replaced by Versene and suggested that the function of the glycine was not for protein synthesis but to chelate ions of copper or zinc which might have contaminated the medium. However, Brin-read more
Citations
More filters
Journal ArticleDOI
Development of preimplantation embryos of the golden hamster in a defined culture medium.
TL;DR: The data suggest that an important metabolic change may occur in hamster embryos during a critical period at the 8-cell stage of development, which is an approach towards achieving complete preimplantation development ofHamster embryos in vitro.
Journal ArticleDOI
In vitro development of bovine one-cell embryos: Influence of glucose, lactate, pyruvate, amino acids and vitamins
Y. Takahashi,N. L. First +1 more
TL;DR: It is demonstrated that a semi-chemically defined medium can successfully support the development of bovine embryos to the morula stage to a limited extent, but the medium lacks some nutrients or growth factors to fully support development through the blastocyst stage.
Book ChapterDOI
Nutrient requirements for the culture of preimplantation embryos in vitro
TL;DR: All normal mouse embryos develop from the pronuclear stage to blastocysts when cultured in a simple chemically defined medium and a high proportion of these develop into normal mice if introduced into the uteri of pseudopregnant females.
Journal ArticleDOI
Aspects of in vitro fertilization and embryo culture in domestic animals.
TL;DR: An examination of the spermatozoa and oocyte incubation media showed no media or protein supplement to be superior in promoting in vitro fertilization in cattle, sheep or swine.
Journal ArticleDOI
Chemically defined media and the culture of mammalian preimplantation embryos: historical perspective and current issues
TL;DR: The history of the development of chemically defined media for the culture of preimplantation embryos is detailed to detail and the adaptations to stress that occur when embryos are placed in a chemically defined environment are reviewed.
References
More filters
Book ChapterDOI
Nutrient requirements for the culture of preimplantation embryos in vitro
TL;DR: All normal mouse embryos develop from the pronuclear stage to blastocysts when cultured in a simple chemically defined medium and a high proportion of these develop into normal mice if introduced into the uteri of pseudopregnant females.
Journal ArticleDOI
Complete development in vitro of the pre-implantation stages of the mouse in a simple chemically defined medium
W. K. Whitten,J. D. Biggers +1 more
TL;DR: A simple chemically defined medium in which the eggs of hybrid mice develop from the 1-cell stage to the blastocyst completely in vitro is described, which is capable of implantation and thus has overcome the final barrier which prevented complete development in vitro of the pre-implantation stages of a mammal independent of the female genital tract.
Journal ArticleDOI
Protein content of the mouse embryo during the first five days of development
TL;DR: Protein content and net protein synthesis of the pre-implantion mouse embryo are determined to determine for the interpretation of respiratory studies, and for interpreting in-vitro culture information and autoradiographie information dealing with protein metabolism in the early mouse embryo.
Journal ArticleDOI
Studies on the development of mouse embyros in vitro. III. The effect of fixed-nitrogen source
TL;DR: It was found that two-cell mouse ova would not develop into blastocysts in medium that did not contain a fixed-nitrogen source, and single amino acids were omitted from the medium containing the constituent amino acids of BSA in an attempt to demonstrate an essential amino acid requirement of two- cell mouse Ova.
Related Papers (5)
Complete development in vitro of the pre-implantation stages of the mouse in a simple chemically defined medium
W. K. Whitten,J. D. Biggers +1 more