Effect of environmental parameters on the biodegradation of oil sludge.
J T Dibble,R Bartha +1 more
TLDR
Oil sludge biodegradation was optimal at a soil water-holding capacity of 30 to 90%, a pH of 7.5 to 7.8, C:N and C:P ratios of 60:1 and 800:1, respectively, and a temperature of 20 degrees C or above.Abstract:
A laboratory study was conducted with the aim of evaluating and optimizing the environmental parameters of "landfarming", i.e., the disposal by biodegradation in soil of oily sludges generated in the refining of crude oil and related operations. Oil sludge biodegradation was monitored by CO2 evolution and by periodic analysis of residual hydrocarbons. The parameters studied were soil moisture, pH, mineral nutrients, micronutrients, organic supplements, treatment rate, teratment frequency, and incubation temperature. Oil sludge biodegradation was optimal at a soil water-holding capacity of 30 to 90%, a pH of 7.5 to 7.8, C:N and C:P ratios of 60:1 and 800:1, respectively, and a temperature of 20 degrees C or above. Addition of micronutrients and organic supplements was not beneficial; sewage sludge interfered with hydrocarbon biodegradation. Breakdown of the saturated hydrocarbon (alkane and cycloalkane) fraction was the highest at low application rates, but higher application rates favored the biodegradation of the aromatic and asphaltic fractions. An application rate of 5% (wt/wt) oil sludge hydrocarbon to the soil (100,000 liters/hectare) achieved a good compromise between high biodegradation rates and efficient land use and resulted in the best overall biodegradation rate of all hydrocarbon classes. Frequent small applications resulted in higher biodegradation than single large applications. Two 100,000-liter/hectare (255 barrels per acre) or four 50,000-liter/hectare oil sludge hydrocarbon applications per growing season seem appropriate for most temperate zone disposal sites.read more
Citations
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Journal ArticleDOI
Microbial degradation of hydrocarbons in the environment.
J G Leahy,Rita R. Colwell +1 more
TL;DR: Rates of biodegradation depend greatly on the composition, state, and concentration of the oil or hydrocarbons, with dispersion and emulsification enhancing rates in aquatic systems and absorption by soil particulates being the key feature of terrestrial ecosystems.
Journal ArticleDOI
Microbial degradation of petroleum hydrocarbons: an environmental perspective.
Journal ArticleDOI
Bioremediation of soil contaminated with polynuclear aromatic hydrocarbons (PAHs): A review
Susan C. Wilson,Kevin C. Jones +1 more
TL;DR: The bioremediation of contaminated soils with in-situ, on-site, and bioreactor techniques is reviewed, together with the factors affecting PAH degradation.
Journal ArticleDOI
Bioaugmentation and biostimulation strategies to improve the effectiveness of bioremediation processes.
TL;DR: The present review discusses the effectiveness as well as the limitations of bioaugmentation and biostimulation processes for in situ bioremediation of accidental spills and chronically contaminated sites worldwide.
Book ChapterDOI
Hydrocarbon Biodegradation and Oil Spill Bioremediation
Ronald M. Atlas,Richard Bartha +1 more
TL;DR: Applied studies focused on optimizing microbial growth on low- to middle-molecular-weight hydrocarbons and developed fermentor designs for large-scale single cell protein production with agitation and aeration systems that permitted high rates of microbes growth on soluble and highly emulsified hydrocarbon substrates.
References
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Journal ArticleDOI
Features of a Flask and Method for Measuring the Persistence and Biological Effects of Pesticides in Soil
Richard Bartha,David Pramer +1 more
TL;DR: Barbela et al. as discussed by the authors presented a Flask and method for measuring the persistence and biological effects of pesticides in the soil, and used it to measure the effect of pesticides.
Journal ArticleDOI
Degradation and mineralization of petroleum in sea water: limitation by nitrogen and phosphorous.
Ronald M. Atlas,Richard Bartha +1 more
TL;DR: Biodegradation and mineralization of petroleum, added at 1% (v/v) to freshly collected sea water, were measured using gas–liquid chromatographic, residual weight, and CO2‐evolution techniques.
Journal ArticleDOI
Microbial Utilization of Crude Oil
TL;DR: The utilization of two crude oil samples of different quality at 4 and 30 C has been studied by using pure and mixed bacterial cultures obtained by enrichment procedures, showing that the n-saturate fraction had been preferentially used.
Microbial Degradation of Petroleum in the Marine Environment.
TL;DR: It was found that naphthalene-degrading bacteria rapidly colonized new wood pilings within hours after installation but that the rate of attachment and subsequent colonization was significantly slower for the naphthaene- enriched creosote-treated wood.
Book ChapterDOI
The Microbiology of Aquatic Oil Spills
Richard Bartha,Richard Bartha +1 more
TL;DR: This chapter discusses the microbiology of aquatic oil spills and illustrates the effects of petroleum hydrocarbons on microorganisms, microbial emulsification and degradation of petroleum, and the microorganisms and oil pollution abatement.