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Showing papers in "Microbiological Research in 1990"


Journal ArticleDOI
TL;DR: The physiological functions of PHB as a reserve material and in symbiotic nitrogen fixation and its presence in bacterial plasma membranes and putative role in transformability and calcium signaling are also considered.

2,654 citations


Journal ArticleDOI
TL;DR: Rates of biodegradation depend greatly on the composition, state, and concentration of the oil or hydrocarbons, with dispersion and emulsification enhancing rates in aquatic systems and absorption by soil particulates being the key feature of terrestrial ecosystems.

2,450 citations


Journal ArticleDOI
B J Bachmann1
TL;DR: The linkage map of Escherichia coli K-12 depicts the arrangement of genes on the circular chromosome of this organism and there are now 1,403 loci placed on the linkage group, which may represent between one-third and one-half of the genes in this organism.

877 citations


Journal ArticleDOI
TL;DR: These mutants show a remarkable degree of genetic overlap in various vacuolar functions, suggesting that these functions are not individual, discrete properties of the vacuole but, rather, are closely interrelated.

771 citations


Journal ArticleDOI
TL;DR: It is concluded that so far neither experimental nor theoretical evidence exists for a direct influence of interfaces on microbial activity.

732 citations


Journal ArticleDOI
TL;DR: A collection of nuclear respiratory-defective mutants (pet mutants) of Saccharomyces cerevisiae consisting of 215 complementation groups is described and an up-to-date list of the known genes coding for mitochondrial constituents and for proteins whose expression is vital for the respiratory competence of S. Cerevisiae is cataloged.

539 citations


Journal ArticleDOI
TL;DR: A popular interpretation of the major codon preference is that it reflects the operation of a regulatory device that controls the expression of individual proteins, but this widely accepted model is not supported by kinetic theory or by experimental results.

474 citations


Journal ArticleDOI
TL;DR: The single-stranded DNA-binding protein (SSB) of Escherichia coli is involved in all aspects of DNA metabolism: replication, repair, and recombination.

467 citations


Journal ArticleDOI
TL;DR: Using a number of in vitro assays, researchers have been able to elucidate the action mechanism of the UvrABC nuclease complex, which represents a unique form of protein-DNA interaction.

402 citations


Journal ArticleDOI
S Krawiec1, M Riley1
TL;DR: A model depicting the dynamics of the evolution and genetic activity of the bacterial chromosome is described which entails acquisition by recombination of clonal segments within the chromosome, consistent with only a few genetic types of E. coli worldwide.

381 citations


Journal ArticleDOI
TL;DR: These social behaviors indicate that myxobacterial colonies are not merely collections of individual cells but are societies in which cell behavior is synchronized by cell-cell interactions.

Journal ArticleDOI
B L Trumpower1
TL;DR: The cytochrome bc1 complex is the most widely occurring electron transfer complex capable of energy transduction and the mechanism which links proton translocation to electron transfer through these proteins appears to be universal to all bc1 complexes.

Journal ArticleDOI
TL;DR: It is shown that this collective behavior of water molecules amounts to a considerable biological force, which can be equivalent to a pressure of 1,000 atm (1.013 x 10(5) kPa), and suggested that cells selectively accumulate K+ ions and compatible solutes to avoid extremes of water structure in their aqueous compartments.

Journal ArticleDOI
TL;DR: Although most molecular analysis to data has been with C. albicans, the same methodologies are proving highly effective with other Candida species, seeing increased application to biological questions such as drug resistance, virulence determinants, and the phenomenon of phenotypic variation.

Journal ArticleDOI
TL;DR: A model for replication and integration of invertrons is presented, as well as a model for transposition of transposable elements.

Journal ArticleDOI
TL;DR: It is proposed that the degree of subsaturation of the macromolecular biosynthetic apparatus renders a variable fraction of RNA polymerase and ribosomes unavailable for the initiation of new chain synthesis and that this, at least in part, determines the composition of the cell as a function of the growth rate.

Journal ArticleDOI
TL;DR: The objective of this article is to survey what has been learned of morphogenesis of walled eucaryotic microorganisms as a set of problems in cellular heredity, biochemistry, physiology, and organization.

Journal ArticleDOI
TL;DR: Despite the biochemical complexity exhibited by Pi-linked antiporters, they resemble all other secondary carriers at a molecular level and show a likely topology in which two sets of six transmembrane alpha-helices are connected by a central hydrophilic loop.

Journal ArticleDOI
TL;DR: Current knowledge of the distribution, structure, and biological role(s) of the TraT protein is considered, and comparison of the corresponding amino acid sequences suggests that a central region of five amino acid residues flanked by hydrophobic domains determines the specificity of the protein in surface exclusion.

Journal ArticleDOI
TL;DR: The chemiosmotic model of energy transduction offers a satisfying and widely confirmed understanding of the action of uncouplers on such processes as oxidative phosphorylation; the uncoupler, by facilitating the transmembrane movement of protons or other compensatory ions, reduces the electrochemical proton gradient that is posited as the energy intermediate for many kinds of bioenergetic work.

Journal ArticleDOI
TL;DR: Biological studies of the scrapie agent suggest that a nucleic acid may be involved in the disease, but sensitive molecular biology techniques have yet to identify this putative nucleic acids.

Journal ArticleDOI
TL;DR: The glutamine cycle is not futile, because it is necessary to drive an effective carbon flow to support growth and, in doing so, buffers variations in the nutrient supply and drives energy generation and carbon flow for optimal cell function.