Journal ArticleDOI
Effects of an injectable platelet-rich fibrin on osteoblast behavior and bone tissue formation in comparison to platelet-rich plasma
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TLDR
The results showed that all cells had high survival rates throughout the entire study period irrespective of culture-conditions, and favored the use of the naturally-formulated i-PRF when compared to traditional PRP with anti-coagulants.Abstract:
Platelet-rich plasma (PRP) has been utilized for many years as a regenerative agent capable of inducing vascularization of various tissues using blood-derived growth factors. Despite this, drawbacks mostly related to the additional use of anti-coagulants found in PRP have been shown to inhibit the wound healing process. For these reasons, a novel platelet concentrate has recently been developed with no additives by utilizing lower centrifugation speeds. The purpose of this study was therefore to investigate osteoblast behavior of this novel therapy (injectable-platelet-rich fibrin; i-PRF, 100% natural with no additives) when compared to traditional PRP. Human primary osteoblasts were cultured with either i-PRF or PRP and compared to control tissue culture plastic. A live/dead assay, migration assay as well as a cell adhesion/proliferation assay were investigated. Furthermore, osteoblast differentiation was assessed by alkaline phosphatase (ALP), alizarin red and osteocalcin staining, as well as re...read more
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Journal ArticleDOI
Injectable platelet rich fibrin: cell content, morphological, and protein characterization.
Hugo de Almeida Varela,Júlio C.M. Souza,Rubens M. Nascimento,Raimundo Fernandes de Araújo,Roseane Carvalho Vasconcelos,Rômulo S. Cavalcante,Paulo Marcos da Matta Guedes,Aurigena Antunes de Araújo +7 more
TL;DR: The use of an injectable platelet rich fibrin can be extended to other applications in the field of orthopedics, periodontics, and implant dentistry on the repairing process of both soft and mineralized tissues.
Journal ArticleDOI
Titanium mesh for bone augmentation in oral implantology: current application and progress.
TL;DR: Guided bone regeneration (GBR) is an effective and simple method for bone augmentation, which is often used to reconstruct the alveolar ridge when the bone defect occurs in the implant area as mentioned in this paper.
Journal ArticleDOI
Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies
Franz Josef Strauss,Jila Nasirzade,Zahra Kargarpoor,Alexandra Stähli,Alexandra Stähli,Reinhard Gruber,Reinhard Gruber +6 more
TL;DR: The overall findings suggest that PRF induces cell proliferation, migration, adhesion, and differentiation along with possessing anti-inflammatory properties further supporting its therapeutic potential in wound healing and bone regeneration.
Journal ArticleDOI
Injectable-platelet rich fibrin using the low speed centrifugation concept improves cartilage regeneration when compared to platelet-rich plasma.
Mustafa Abd El Raouf,Xuzhu Wang,Si Miusi,Jihua Chai,Abdel Basit Mohamed AbdEl-Aal,Mekkawy M. Nefissa Helmy,Shahram Ghanaati,Joseph Choukroun,Elisa Choukroun,Yufeng Zhang,Richard J. Miron +10 more
TL;DR: The use of i-PRF using the low speed centrifugation concept significantly promoted chondrocyte activity and further improved cartilage regeneration when compared to PRP.
Journal ArticleDOI
Gelatin Nanoparticle-Injectable Platelet-Rich Fibrin Double Network Hydrogels with Local Adaptability and Bioactivity for Enhanced Osteogenesis
Zhixiang Mu,Kaiwen Chen,Shuai Yuan,Yihan Li,Yuanding Huang,Chao Wang,Yang Zhang,Wenzhao Liu,Wenping Luo,Panpan Liang,Xiaodong Li,Jinlin Song,Ping Ji,Fang Cheng,Huanan Wang,Tao Chen +15 more
TL;DR: This adaptable and bioactive DN hydrogel can achieve enhanced bone regeneration in treating complex bone defects by maintaining long‐term bone mass and withstanding the functional mechanical stimuli.
References
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Journal ArticleDOI
Platelet-rich plasma: Growth factor enhancement for bone grafts
Robert E. Marx,Eric R. Carlson,Ralph M. Eichstaedt,Ralph M. Eichstaedt,Steven R Schimmele,Steven R Schimmele,James E. Strauss,Karen R. Georgeff +7 more
TL;DR: Monoclonal antibody assessment of cancellous cellular marrow grafts demonstrated cells that were capable of responding to the growth factors by bearing cell membrane receptors and evidenced a radiographic maturation rate 1.62 to 2.16 times that of grafts without platelet-rich plasma.
Journal ArticleDOI
Platelet-Rich Plasma From Basic Science to Clinical Applications
TL;DR: This study reviews and evaluates the human studies that have been published in the orthopaedic surgery and sports medicine literature and the regulation of PRP by antidoping agencies is discussed.
From Basic Science to Clinical Applications
Journal ArticleDOI
A comparative study of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro.
TL;DR: PRF released autologous growth factors gradually and expressed stronger and more durable effect on proliferation and differentiation of rat osteoblasts than PRP in vitro, and is superior to PRP, from the aspects of expression of ALP and induction of mineralization.
Journal ArticleDOI
The cell biology of leukocyte-mediated proteolysis.
TL;DR: This work has identified several mechanisms by which inflammatory cells can degrade extracellular proteins in a milieu that contains high‐affinity proteinase inhibitors.