Journal ArticleDOI
Effects of pH and organic solvent on chromatographic behavior in capillary electrochromatography
Shinya Kitagawa,Takao Tsuda +1 more
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In this paper, the separation of solutes due to their different mobilities is obtained by the application of high voltage along a 50 μm i.d. capillary column.Abstract:
Electrochromatography is demonstrated using a 50 μm i.d. capillary column. The separation of solutes due to their different mobilities is obtained by the application of high voltage along the column. The elution times of solutes are dependent on the pH and composition of the eluent. Both electrophoretic and electroosmotic flow velocities are almost constant between 30 and 90% methanol in the eluent, and they increase with more than 90% methanol. The pH dependence of the electroosmotic flow velocity may be related to the dissociation of unreacted silanol groups on the surface of the ODS-silica particles.read more
Citations
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The efficient analysis of neutral and highly polar pharmaceutical compounds using reversed-phase and ion-exchange electrochromatography
N. W. Smith,M. B. Evans +1 more
TL;DR: In this article, a strong cation exchanger in place of C18-silica was used to resolve a series of highly basic compounds with very high efficiencies, with very little evidence of peak tailing.
Journal ArticleDOI
Electrochromatography in chemically modified etched fused-silica capillaries
Joseph J. Pesek,Maria T. Matyska +1 more
TL;DR: A new method of electrochromatography is described in which a 50-microns capillary is etched with ammonium hydrogen difluoride, followed by modification of the new surface via a silation reaction with triethoxysilane to produce a hydride intermediate, and subsequently subjected to hydrosilation using 1-octadecene.
Journal ArticleDOI
Capillary electrochromatography of proteins and peptides with porous-layer open-tubular columns.
TL;DR: The overall migration behavior of the four proteins was the result of an interplay of chromatographic retention and electrophoretic migration, and was different from that observed in capillary zone electrophoresis or in reversed-phase chromatography under similar conditions.
Journal ArticleDOI
Theory of capillary electrochromatography.
Keith D. Bartle,Peter Myers +1 more
TL;DR: The present state of the theory of capillary electrochromatography is reviewed, and emphasis is placed on electroosmosis and the electrical double layer, and the generally good understanding of the factors affecting the Electroosmotic flow in CEC columns.
Journal ArticleDOI
Capillary electrochromatography: A review
TL;DR: Capillary electrochromatography (CEC) is a modern liquid chromatographic technique which has attracted renewed curiosity from the scientific community in recent years, despite having origins in the 1970s as discussed by the authors.
References
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Journal ArticleDOI
Free zone electrophoresis
TL;DR: The free zone electrophoresis method described in this monograph can be used for the fractionation of small molecules, large molecules, and particles, and its versatility is illustrated in runs with inorganic ions, organic ions, nucleic acid bases, nucleosides, nucleotides, proteins,ucleic acids, subcellular particles, viruses, and erythrocytes.
Journal ArticleDOI
Electrochromatography in packed tubes using 1.5 to 50 μm silica gels and ODS bonded silica gels
John H. Knox,I. H. Grant +1 more
TL;DR: It is shown that, following theory, there is no evidence of any reduction in electroosmotic velocity in columns packed with particles down to 1.5 μm diameter, and that reduced plate heights as low as unity are attainable for unretained solutes.
Journal ArticleDOI
Open-tubular microcapillary liquid chromatography with electro-osmosis flow using a UV detector
TL;DR: In this article, the linear velocity of electro-osmosis in a capillary tube 30 ×90 cm x 30 −200 μm I.D. was measured, and the physical and geometrical parameters of EOSMosis and its use were discussed.
Journal ArticleDOI
Electroosmotic propulsion of eluent through silica-based chromatographic media
Journal ArticleDOI
Chromatographic behavior in electrochromatography
TL;DR: In this paper, an equation for the height equivalent of a theoretical plate in electrochromatography is developed, which is the summation of pressurized flow and electrophoretic flow.
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Electrochromatography in packed tubes using 1.5 to 50 μm silica gels and ODS bonded silica gels
John H. Knox,I. H. Grant +1 more