Journal ArticleDOI
Energy-dependent transformation of mouse gall bladder epithelial cells in a Ca2+-depleted medium.
Toyoshi Fujimoto,Kazuo Ogawa +1 more
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The results indicate that in the Ca 2+ -free media, mouse gall bladder epithelia go through a drastic transformation process that is energy dependent and is mediated by microfilaments.About:
This article is published in Journal of Ultrastructure Research.The article was published on 1982-06-01. It has received 13 citations till now. The article focuses on the topics: Apical cytoplasm & Tight junction.read more
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Participation of plasma membrane proteins in the formation of tight junction by cultured epithelial cells
TL;DR: Observations suggest that proteins are involved in tight junction formation, which consists of separable synthetic and assembly phases and can be synthesized without intercellular contacts or cell-substratum attachment.
Journal ArticleDOI
Chapter 10 Improved methodology for cell culture of human and monkey retinal pigment epithelium
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Uptake, processing and release of retinoids by cultured human retinal pigment epithelium.
John G. Flannery,William T. O'Day,William T. O'Day,Bruce A. Pfeffer,Joseph Horwitz,Dean Bok,Dean Bok +6 more
TL;DR: Results demonstrate retinoid isomerase, retinol oxidoreductase and retinyl ester synthetase activity in human RPE cells cultured under the appropriate conditions and show that IRBP can serve as a carrier ofretinol through an aqueous medium to the RPE, and illustrate that the visual cycle can be studied in vitro.
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Evidence that a Ca2+ chelator and a calmodulin blocker interfere with the structure of inter-Sertoli junctions.
Emilia Franchi,Marina Camatini +1 more
TL;DR: In vitro, junction integrity appears to be related to Ca2+ level, andCa2+ depletion apparently interferes with Ca2+.
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Intercellular coupling in frog heart muscle. Electrophysiological and morphological aspects.
TL;DR: It is concluded that the junctions observed in frog atrial muscle are analogous to gap junctions of insect or mammalian cells in spite of the different size and arrangement of the particles.
References
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A low-viscosity epoxy resin embedding medium for electron microscopy.
TL;DR: A low-viscosity embedding medium based on ERL-4206 is recommended for use in electron microscopy and has a long pot life of several days and infiltrates readily because of its low viscosity.
Book ChapterDOI
Structure and function of intercellular junctions.
TL;DR: This chapter reviews the morphological information on intercellular junctions derived from thin-sectioning, negative staining and freeze-cleave techniques, as well as from x-ray diffraction and biochemical investigations, and correlates the structural parameters with known or proposed physiological functions.
Journal ArticleDOI
Fracture faces of zonulae occludentes from "tight" and "leaky" epithelia
TL;DR: Epithelia of intermediate permeabilities exhibited junctions with intermediate or variable morphology, finding that the zonula occludens from a "very leaky" epithelium, the proximal convoluted tubule of the mouse kidney, is extremely shallow in the apical-basal direction, and consists of five or more interconnected junctional strands interposed between luminal and lateral membrane surfaces.
Journal ArticleDOI
Transmembrane control of the receptors on normal and tumor cells: I. Cytoplasmic influence over cell surface components☆
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Polarized monolayers formed by epithelial cells on a permeable and translucent support.
TL;DR: Measurements of Na+ permeability of electrical and tracer methods ruled out exchange diffusion as a mechanism for ion permeation and the lack of current saturation in the I/deltapsi curves does not support the involvement of carriers.