Fertility of bull semen at high extension rates in Tris-buffered extenders.
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TLDR
A 0.20m Tris-yolk extender buffered to pH 6.50 or 6.75 was superior to other combinations tested in preserving motility of sperm as mentioned in this paper.About:
This article is published in Journal of Dairy Science.The article was published on 1970-10-01 and is currently open access. It has received 83 citations till now. The article focuses on the topics: Sperm washing & Sperm.read more
Citations
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Journal ArticleDOI
Storage of bovine semen in liquid and frozen state
R. Vishwanath,P. Shannon +1 more
TL;DR: The historical and current methods used for storage of bovine semen are described and the essential physiological differences between liquid and frozen semen are addressed.
Journal ArticleDOI
Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility
TL;DR: The results indicate a certain proportion of bull spermatozoa express PS on their surface after thawing, and that the incidence of such cells is inversely correlated to sperm viability, and positively correlated to abnormal sperm chromatin condensation since they eventually undergo necrosis.
Journal ArticleDOI
Relationships between computerized measurements of motion of frozen-thawed bull spermatozoa and fertility.
TL;DR: A computerized system (CellSoft, CRYO Resources, Ltd.) was validated using video tapes of frozen-thawed bull spermatozoa diluted in filtered egg yolk-citrate extender and analyzed at 30 frames/sec for the percentage of motile spermutozoa and linear velocity and may facilitate prediction of the relative fertility of bull spermarozoa.
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Cryopreservation of flow-sorted bovine spermatozoa.
TL;DR: Fertilizing capacity of flow-sorted sperm is quite acceptable as predicted by simple laboratory assays, and sexed bovine sperm for commercial AI may be available within 2 years.
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Assessment of sperm quality through fluorometry and sperm chromatin structure assay in relation to field fertility of frozen-thawed semen from Swedish AI bulls
A. Januskauskas,A. Januskauskas,Anders Johannisson,Anders Johannisson,Heriberto Rodriguez-Martinez,Heriberto Rodriguez-Martinez +5 more
TL;DR: The results indicate that fluorometric assessment is in good agreement with other practiced procedures and can be performed with sufficient accuracy and may be a valuable complement for routinely practiced microscopic evaluation of sperm morphology of AI bull semen.
References
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Journal ArticleDOI
Livability of Bovine Spermatozoa at 5, −25, and −85 C in Tris-Buffered and Citrate-Buffered Yolk-Glycerol Extenders
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Osmotic Pressure and pH Effects on Survival of Frozen Bovine Spermatozoa
J. Steinbach,Robert H. Foote +1 more
TL;DR: In this article, sperm from ten bulls was extended in egg yolk extenders buffered with live concentrations of Tris adjusted to four pH levels, and containing two levels of glycerol, to study their interactions during semen freezing.
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Factors Influencing Metabolic Activity of Bull Spermatozoa. III. pH
G.W. Salisbury,W.C. Kinney +1 more
TL;DR: P pH per se did not influence the respiratory quotients obtained, nor was there a marked effect of pH on oxygen uptake of the sperm cells, though it tended to be highest at the higher pH levels; however, pH did influence markedly aerobic fructolysis.
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Fertility of Bull Semen Stored Up to Four Days at 5° C. In 20% Egg Yolk Extenders
TL;DR: The adoption of CUE for routine use by the local artificial breeding cooperative resulted in an immediate and consistent increase of about two percentage units, bringing the 60- to 90-day nonreturn rate to an average of 74.9% for nearly 300,000 first inseminations.