Journal ArticleDOI
Formation of sulfhydryl groups in the culture medium by human diploid fibroblasts
Shiro Bannai,Tetsuro Ishii +1 more
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When human diploid fibroblasts IMR‐90 are cultured in routinely used medium (Eagle's basal medium supplemented with 10% fetal calf serum), sulfhydryl compounds appear in the medium, and it is shown that a part of medium cystine is converted into cysteine by the cells.Abstract:
When human diploid fibroblasts IMR-90 are cultured in routinely used medium (Eagle's basal medium supplemented with 10% fetal calf serum), sulfhydryl compounds appear in the medium. The major component of these sulfhydryl compounds is cysteine, and it is shown that a part of medium cystine is converted into cysteine by the cells. It is also shown that the sulfhydryl groups of serum albumin, which are masked and barely detectable before the culture, are restored. Probably cysteine formed by the cells reacts with serum albumin to give rise to the protein sulfhydryl groups via sulfhydryl–disulfide exchange reactions. Total sulfhydryl concentrations in the medium are maintained in a considerable level throughout the culture, and a possible physiological function of these sulfhydryl groups is discussed.read more
Citations
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Oxidized redox state of glutathione in the endoplasmic reticulum
TL;DR: Results suggest that the demonstrated preferential transport of GSSG compared to GSH into the ER lumen may contribute to this redox compartmentation.
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Superoxide and hydrogen peroxide in relation to mammalian cell proliferation
TL;DR: A wide variety of normal and malignant cell types generate and release superoxide or hydrogen peroxide in vitro either in response to specific cytokine/growth factor stimulus or constitutively in the case of tumour cells.
Journal ArticleDOI
Maintenance of neuronal glutathione by glial cells.
TL;DR: It is suggested that neurons maintain their glutathione level by taking up cysteine provided by glial cells, because the culture medium contains cystine, but not cys‐teine.
Journal ArticleDOI
Transport of cystine and cysteine in mammalian cells.
TL;DR: Synthesis of cystine as y-glutamyl peptide and its use as a building block for peptide synthesis is continuing to be studied.
Journal ArticleDOI
Mechanism of growth stimulation of L1210 cells by 2-mercaptoethanol in vitro. Role of the mixed disulfide of 2-mercaptoethanol and cysteine.
Tetsuro Ishii,S Bannai,Y Sugita +2 more
TL;DR: The mechanism of the growth-promoting action of 2-mercaptoethanol on mouse lymphoma L1210 cells in vitro has been studied and it is shown that the cells are able to utilize cystine constantly.
References
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Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin
James R. Benson,P. E. Hare +1 more
TL;DR: O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products that can be detected easily and soluble and stable in aqueous buffers.
Journal ArticleDOI
Reactions of N-ethylmaleimide with peptides and amino acids
TL;DR: The present paper gives the rates of reaction of NEM with the ac-amino group of glycylalanine, with the N-terminal peptide of the a-chain of haemoglobin and with theN-terminals of the ,B- chain of haenoglobin.
Journal ArticleDOI
Characterization of a new human diploid cell strain, IMR-90.
Warren W. Nichols,D. G. Murphy,Vincent J. Cristofalo,Lorraine H. Toji,A. E. Greene,S. A. Dwight +5 more
TL;DR: A new human diploid fibroblast-like cell line has been established from lung tissue of a female fetus that has been frozen away in large quantity and characterized for use in research and related purposes.
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