Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TLDR
The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.Abstract:
Summary
The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.read more
Citations
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
Journal ArticleDOI
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
H. Schägger,G. von Jagow +1 more
TL;DR: A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described, and the omission of glycine and urea prevents disturbances which might occur in the course of subsequent amino acid sequencing.
References
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Journal ArticleDOI
Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults.
Book ChapterDOI
Zone electrophoresis in starch gels and its application to studies of serum proteins.
TL;DR: The starch-gel electrophoretic method can be applied to the investigation of many complex protein mixtures in addition to serum proteins, for example, enzymes, hormones, and tissue extracts.
Journal ArticleDOI
Acrylamide Gel as a Supporting Medium for Zone Electrophoresis
Samuel Raymond,Lewis Weintraub +1 more
TL;DR: Acrylamide polymerized in buffer solutions forms a stable, flexible, transparent gel which is useful in zone electrophoresis.
Related Papers (5)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more