Glass capillary gas chromatography of secondary amines in foods with flame photometric detection after derivatization with benzenesulfonyl chloride

TLDR: A simple and selective gas chromatographic method was established for determining naturally occurring secondary amines as mentioned in this paper, which was carried out with a capillary coated with OV-101 and a flame photometric detector.

Abstract: A simple and selective gas chromatographic method was established for determining naturally occurring secondary amines. Secondary amines separated from foods by extraction with dichloro-methane and reextraction with hydrochloric acid were readily converted into the corresponding sulfonamides by reaction with benzenesulfonyl chloride under alkaline condition. Gas chromatography was carried out with a capillary coated with OV-101 and a flame photometric detector. Column temperature was programmed from 170 to 230°C at a rate of 5°C/min.The obtained sulfonamides were separated from one another within 40 min.Eleven secondary amines examined were added to 5 kinds of foods and recovered from them. The mean recovery rates were in the range 71.3% (dimethylamine)–99.8% (piperidine).The limits of detection varied from 0.002 ppm (dimethylamine) to 0.01 ppm (morpholine).