Growth of Azotobacter vinelandii on Soil Nutrients.
TLDR
Azotobacter vinelandii cells grew well in a medium made from soil and distilled water which contained little or no carbohydrate, but cell morphology was different from that usually seen in chemically defined, nitrogen-free media which contain glucose.Abstract:
Azotobacter vinelandii cells grew well in a medium made from soil and distilled water which contained little or no carbohydrate. They utilized p-hydroxybenzoic acid and other phenolic acids, soil nitrogen, and water-soluble mineral substances. Seventeen soils which supported excellent growth of A. vinelandii contained 11 to 18 different phenolic acids each, including p-hydroxybenzoic, m-hydroxybenzoic, vanillic, p-coumeric, syringic, cis- and trans-ferrulic, and other unidentified aromatic acids. Three white, chalky "caliche" soils which were taken from areas where no plants grew failed to support the growth of A. vinelandii, and these contained no, two, and three phenolic acids, respectively. A. vinelandii did not fix nitrogen when growing in dialysates of soils which contained numerous phenolic acids. Growth was ample and rapid in most of the soils tested, but cell morphology was different from that usually seen in chemically defined, nitrogen-free media which contain glucose.read more
Citations
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The Estimation of the Bactericidal Power of the Blood. With a Note by J. O. IRWIN.
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TL;DR: The survival rate of Staph. aureus in a standard volume of denbrinated blood is a reliable quantitative measure of the bactericidal power of blood, and the number of viable organisms in the inoculum and in the blood-bacterium mixture may be estimated with the necessary accuracy by counts of colonies developing from measured volumes of the fluids let fall on to the surface of solid media as mentioned in this paper.
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Decomposition and biomass incorporation of 14c-labeled glucose and phenolics in taiga forest floor: effect of substrate quality, successional state, and season
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TL;DR: In this paper, forest floor samples from early, intermediate and mature successional sites in the taiga of interior Alaska were exposed to 14 C-labeled glucose and two phenolic acids.
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Production of B-group vitamins by two Azotobacter strains with phenolic compounds as sole carbon source under diazotrophic and adiazotrophic conditions.
TL;DR: Two strains ofAzotobacter vinelandii strain ATCC 12837 and A. chroococcum strain H23 were able to grow on N‐free or NH4Cl‐amended chemically‐defined media, with protocatechuic acid or sodium p‐hydroxybenzoate or sodium succinate as sole carbon (C) sources.
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Changes of ploidy during the Azotobacter vinelandii growth cycle.
TL;DR: Growth in minimal medium does not result in the spectacular changes of ploidy observed during rapid growth; this observation suggests that the polyploidy of A. vinelandii may not exist outside the laboratory.
References
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The estimation of the bactericidal power of the blood.
TL;DR: The survival rate, p, of a measured inoculum of Staph.
Journal ArticleDOI
The phenolic acids of human urine; paper chromatography of phenolic acids.
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Nitrogen fixation research: a key to world food?
R. W. F. Hardy,U. D. Havelka +1 more
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Evidence for an alternative nitrogen fixation system in Azotobacter vinelandii.
TL;DR: Under conditions of molybdenum deprivation, Nif- mutant strains of several different phenotypic classes underwent phenotypesic reversal to Nif+, as shown by their ability to incorporate 15N2 and to grow in N-free media.
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Regulation of Nitrogen Fixation by Fe‐S Protein II in Azotobacter vinelandii
TL;DR: The time course of oxidation of substrate-amounts of A. vinelandii flavodoxin hydroquinone by catalytic amounts of crude nitrogenase complex shows three characteristic phases: an initial lag phase, a phase with constant rate over a range of redox potentials and a final phase with rapidly declining rate.