Hyaluronidase-sensitive halos around adherent cells. Their role in blocking lymphocyte-mediated cytolysis.
W. H. McBRIDE,J. B. L. Bard +1 more
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A variety of adherent sarcoma, carcinoma and normal cells are surrounded in vitro by thick, transparent zones that spleen cells and a variety of other cells and particles cannot penetrate, which may have general relevance to the in vitro and in vivo killing of tumor cells by immune effector cells.Abstract:
A variety of adherent sarcoma, carcinoma and normal cells are surrounded in vitro by thick, transparent zones (approximately equal to 9 micron thick) that spleen cells and a variety of other cells and particles cannot penetrate. Seven lymphoblastoid cell lines did not possess such halos. The presence of these halos around adherent fibrosarcoma cells appeared to protect them from lymphocyte-mediated cytolysis. Hyaluronidase treatment, which destroyed the halo and allowed lymphocytes to approach the tumor cell membrane, enhanced the cytotoxic action of immune but not of normal spleen cells. These observations, in addition to highlighting a little-known feature of the cell surface, may also be of general relevance to the in vitro and in vivo killing of tumor cells by immune effector cells.read more
Citations
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Hyaluronan fragments: an information-rich system.
TL;DR: The vast range of activities of hyaluronan polymers is reviewed here, in order to determine if patterns can be detected that would provide insight into their production and regulation.
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CD44: Structure, Function and Association with the Malignant Process
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Hyaluronidases: their genomics, structures, and mechanisms of action.
TL;DR: The hyaluronidases from bacteria have been well characterized, and much information is available, but the Hyals in vertebrate tissues, on the other-hand, have not been studied extensively, however, due to the lack of structural information.
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Interactions between Hyaluronan and Its Receptors (CD44, RHAMM) Regulate the Activities of Inflammation and Cancer
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Recent advances in tumor-targeting anticancer drug conjugates.
TL;DR: Recent advances in tumor-targeting drug conjugates including monoclonal antibodies, polyunsaturated fatty acids, folic acid, hyaluronic acid, and oligopeptides are described as tumor- targeting moieties.
References
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Journal ArticleDOI
A rapid method for the isolation of functional thymus‐derived murine lymphocytes
TL;DR: A rapid method is described for effectively removing immunoglobulin‐bearing cells from either primed or unprimed mouse spleen and lymph node cell suspensions.
Journal ArticleDOI
The partition of solutes between buffer solutions and solutions containing hyaluronic acid.
A. G. Ogston,C. F. Phelps +1 more
TL;DR: Holden, H. F, Neumann, W. P. & Habermann, E. (1952).
Journal Article
Immunological Resistance to Pulmonary Metastases in C3Hf/Bu Mice Bearing Syngeneic Fibrosarcoma of Different Sizes
TL;DR: Immunological resistance to pulmonary metastases of a syngeneic methylcholanthrene-induced fibrosarcoma (FSA) was studied in C3Hf/Bu mice that were actively immunized against or that had borne that tumor for varying times and found spleen cells were found to be more effective than lymph node cells.
Journal ArticleDOI
On the pericellular zone of some mammalian cells in vitro
B.J. Clarris,J.R.E. Fraser +1 more
TL;DR: Extensive pericellular zones have been found in cultures of human synovial cells and fibroblasts, but not in some other cell strains, and the potential significance of these zones as a protective barrier is discussed.