Journal ArticleDOI
In vitro propagation of Dipterocarpus alatus and Dipterocarpus intricatus
TLDR
Seedlings were grown in vitro from embryos of Dipterocarpus alatus and D. intricatus and Cotyledonary nodes, excised from the seedlings, were induced to form axillary shoots and in the case of D. elaboratus these could be multiplied rapidly.Abstract:
Seedlings were grown in vitro from embryos of Dipterocarpus alatus and D. intricatus. The problem of explant browning could be overcome by growing embryos initially on a filter paper bridge in liquid medium with activated charcoal. The best basal medium was Woody Plant Medium without the ammonium nitrate. Cytokinin appeared to stimulate seedling growth, 5×10-5 M 2-isopentenyladenine and 10-4 M 6-benzyladenine (BA) being the optimum concentrations for D. alatus and D. intricatus respectively. Cotyledonary nodes, excised from the seedlings, were induced to form axillary shoots and in the case of D. intricatus these could be multiplied rapidly. D. intricatus shoots elongated by reducing the BA level from 10-5 M to 5×10-7 M. Roots developed when shoots were dipped in 10-3 M indolebutyric acid for two minutes and subsequently grown in liquid medium supported by a filter paper bridge.read more
Citations
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The role of activated charcoal in plant tissue culture.
TL;DR: The effect of AC on growth regulator uptake is still unclear but some workers believe that AC may gradually release certain adsorbed products, such as nutrients and growth regulators which become available to plants.
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The use of charcoal in in vitro culture – A review
M. Pan,J. Van Staden +1 more
TL;DR: Its addition to culture medium may promote or inhibit in vitro growth, depending on species and tissues used, and the effects of activated charcoal may be attributed to establishing a darkened environment and adsorption of undesirable/inhibitory substances.
Journal ArticleDOI
Micropropagation of apple — A review
TL;DR: Specific requirements during stages of micropropagation, such as the establishment of in vitro cultures, shoot multiplication, rooting of microshoots and acclimatization are summarized in this review.
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Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview
TL;DR: The present communication attempts to overview the progress in tissue culture, genetic transformation and biotechnological applications in the last decade and future implications.
Journal ArticleDOI
An efficient in vitro method for mass propagation of a woody ornamentalIxora coccinea L.
TL;DR: Stem cuttings were the most suitable explants for multiple-shoot proliferation, and when cultured on a woody plant medium containing 2.5 μM BA produced axillary shoots which branched repeatedly, yielding an average of 27 shoots per explant after 6 weeks in culture.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Book
Plant Propagation by Tissue Culture
TL;DR: The Anatomy and Morphology of Tissue Cultured Plants M.V. Moshkov, G. V. Novikova, M. Stasolla, E. Zazimalova and E.F. George reveal the secrets of successful tissue culture and the challenges faced in implementing and sustaining such a system.
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
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