Journal ArticleDOI
In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture
S. S. Sheelavantmath,Hosakatte Niranjana Murthy,A. N. Pyati,H. G. Ashok Kumar,B. V. Ravishankar +4 more
TLDR
Micropropagation of the endangered terrestrial orchid, Geodorum densiflorum (Lam.) Schltr.Abstract:
Micropropagation of the endangered terrestrial orchid, Geodorum densiflorum (Lam.) Schltr. was achieved through rhizome section culture from in vitro seed- derived rhizomes. Rhizome sections were cultured on Murashige and Skoog (MS) and Knudson C(KC) media supplemented with various growth regulators and 0.1% activated charcoal. The rhizome sections responded on MS medium. Naphthaleneacetic acid (NAA) at 2.0 μM stimulated rhizome growth. However, benzyladenine (BA) at 5.0 μM induced multiple shoots within four weeks of culture and inhibited rhizome growth. The regenerated shoots rooted on MS only or with NAA at 1.0 μM. Well-developed plantlets were transferred to community pots and then to a greenhouse where the plants have been acclimatised.read more
Citations
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The role of activated charcoal in plant tissue culture.
TL;DR: The effect of AC on growth regulator uptake is still unclear but some workers believe that AC may gradually release certain adsorbed products, such as nutrients and growth regulators which become available to plants.
Journal ArticleDOI
Micropropagation of orchids: A review on the potential of different explants
TL;DR: This work endeavours to include the major investigations on explant-based orchid tissue culture starting from the pioneering works of Rotor and Wimber to date to date.
Journal ArticleDOI
The Application of Biotechnology to Orchids
TL;DR: An informative and broad overview of orchid biotechnology, addressing several important aspects such as molecular systematics, modern breeding, in vitro morphogenesis, protoplast culture, flowering control, flower color, somaclonal variation, orchid mycorrhiza, pathogen resistance, virus diagnosis and production of virus-free plants.
Journal ArticleDOI
Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl
TL;DR: Histological observations proved that globular somatic embryos could be produced from the inside and surface of the embryogenic callus during PLB formation.
Journal ArticleDOI
Seed germination and tissue culture of Cymbidium giganteum Wall. ex Lindl.
TL;DR: Although protocorms proliferated profusely in liquid medium, these did not develop further unless transferred to agar-solidified medium within 6–8 weeks, and well-rooted plants were transferred to the greenhouse with 95% survival.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
The effects of 1-naphthaleneacetic acid and N^6-benzyladenine on the growth of Cymbidium forrestii rhizomes in vitro.
Kee-Yoeup Paek,Edward C. Yeung +1 more
TL;DR: An Asiatic orchid was clonally propagated using seed-derived rhizomes as explants and auxin stimulated rhizome growth by increasing branching and fresh weight of the explant, with 1-naphthaleneacetic acid (NAA) being the most effective auxin.
Journal ArticleDOI
Micropropagation of temperate Cymbidium via rhizome culture
Kee-Yoeup Paek,Toyoki Kozai +1 more