Journal ArticleDOI
In vitro regeneration of evergreen azalea from leaves
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TLDR
Rhododendron simsii ‘Hellmut Vogel’ was regenerated using different types of explants, auxins and cytokinins and the duration of the callus induction phase was negatively correlated with the regeneration capacity.Abstract:
Rhododendron simsii ‘Hellmut Vogel’ was regenerated using different types of explants, auxins and cytokinins. After a callus induction phase, with 2,4-dichlorophenoxyacetic acid or α-naphthaleneacetic acid, adventitious shoot regeneration was obtained on a medium supplemented with thidiazuron or zeatin. With thidiazuron shoots were small and a subsequent elongation step was required before rooting. An elongation step was not required when zeatin was used. The duration of the callus induction phase was negatively correlated with the regeneration capacity.read more
Citations
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Journal Article
In vitro Regeneration of Sandal (Santalum album L.) from Leaves
TL;DR: BAP at low concentrations (0.44 and 2.22 µM) was effective in this organogenetic process but exogenous auxin application failed to illicit a similar morphogenetic response.
Journal ArticleDOI
Direct shoot organogenesis from petiole and leaf discs of Withania somnifera (L.) Dunal
TL;DR: An efficient and reproducible procedure is described for direct shoot regeneration using petiole and leaf explants of Withania somnifera (L.) and showed that the shoot buds emerged from sub epidermal parenchymal cells, with no intermediate callus formation.
Journal ArticleDOI
Regeneration of plants from leaflet explants of tissue culture raised safed siris (Albizia procera)
TL;DR: To the authors' knowledge this is the first report of direct regeneration of shoots from leaflet explants in A. procera, and should help facilitate genetic transformation in this species.
Journal ArticleDOI
Somatic polyploid petals: regeneration offers new roads for breeding Belgian pot azaleas
TL;DR: Aiming at introducing polyploidy in the completely diploid breeding collection, in vitro regeneration of tetraploid petal marginal tissue was performed and led to the production of the first induced tetraPloid Belgian azalea, exceptionally interesting for breeding programs.
Journal ArticleDOI
Somatic polyploidy and its consequences for flower coloration and flower morphology in azalea
TL;DR: Neither flower colour pattern nor ploidy differences are periclinal chimeric in origin, but seem to be correlated with the topographic location of the cells within the flower tissue, i.e. the margin of the petals.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal Article
A revised tissue culture medium for shoot multiplication of rhododendron.
TL;DR: Essai biologique en vue d'etudier les effets d'une modification du milieu du Murashige and Skoog sur the multiplication vegetative in vitro du rhododendron.
Journal ArticleDOI
Plant regeneration from leaf explants of Rhododendron ‘P.J.M. Hybrids’
John E. Preece,Miles R Imel +1 more
TL;DR: Pre-culture of leaves on a pre-conditioning medium containing 10 μM indolebutyric acid (IBA) and 50 μM isopentenyladenine (2iP) for 2 weeks before transfer to shoot multiplication medium increased explant survival to greater than 90% as compared with 0–50% when a pre -conditioning treatment was not used.
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