Journal ArticleDOI
In vivo regional levels of PGE and thromboxane in mouse brain: Effect of decapitation, focused microwave fixation, and indomethacin
Raymond F. Anton,Raymond F. Anton,Cleatus Wallis,Cleatus Wallis,Carrie L. Randall,Carrie L. Randall +5 more
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TLDR
The usefulness of the focused microwave irradiation technique for examining in vivo basal prostaglandin levels in mouse brain is confirmed, and PGE levels were significantly higher than TXB2 in all regions.About:
This article is published in Prostaglandins.The article was published on 1983-09-01. It has received 56 citations till now. The article focuses on the topics: Thromboxane B2.read more
Citations
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Alteration of thromboxane and prostacyclin levels in experimental spinal cord injury.
TL;DR: Levels of thromboxane B2 and 6-keto-PGF10 in rabbit spinal cord and cat CSF after impact injury to spinal cord indicate activation of arachidonic acid metabolism and suggests that throm boxane-prostacyclin imbalance may contribute to post-traumatic ischemia.
Journal ArticleDOI
Rat brain arachidonic acid metabolism is increased by a 6-day intracerebral ventricular infusion of bacterial lipopolysaccharide.
Thad A. Rosenberger,Nelly E. Villacreses,Jonathan T. Hovda,Francesca Bosetti,Gayani R. Weerasinghe,Robert N. Wine,G. Jean Harry,Stanley I. Rapoport +7 more
TL;DR: Observations suggest that selective alterations in brain arachidonic acid metabolism involving cytosolic and secretory PLA2 contribute to early pathology in neuroinflammation.
Journal ArticleDOI
LC/MS/MS method for analysis of E2 series prostaglandins and isoprostanes
TL;DR: The method for brain PG analysis provides a high level of selectivity to detect changes in brain PG and isoPG mass under both basal and pathological conditions and reports for the first time the esterified isoPGE2 levels in brain tissue under basal conditions and upon global ischemia.
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An improved LC-MS/MS procedure for brain prostanoid analysis using brain fixation with head-focused microwave irradiation and liquid-liquid extraction
TL;DR: An improved procedure for brain PG analysis provides a reliable, rapid means to detect changes in brain PG mass under both basal and pathological conditions and demonstrates the importance of sample preparation in this process.
Journal ArticleDOI
Postischemic production of eicosanoids in gerbil brain.
TL;DR: The results obtained by ex vivo incubation of tissue slices demonstrate that ischemia and subsequent recirculation cause site-, time- and PG-specific changes of tissue eicosanoid production.
References
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Journal ArticleDOI
Identification of prostaglandin D2 as a major prostaglandin in homogenates of rat brain.
TL;DR: Results indicate that PGD2 is the major cyclooxygenase product in the central nervous system of the rat.
Journal ArticleDOI
Changes in free fatty acids of brain by drug-induced convulsions, electroshock and anaesthesia.
TL;DR: The size of the free fatty acid pool in rat brain was significantly increased following convulsions induced by pentylenetetrazol as well as by electroconvulsive shock.
Journal ArticleDOI
The effect of incomplete cerebral ischemia on prostaglandin levels in rat brain.
E Shohami,J Rosenthal,Lavy S +2 more
TL;DR: It is suggested that the reversibility of cortical activity may be correlated to the accumulation of TXB2 during ischemia and recirculation, and inhibition of its synthesis might improve the post-ischemic reflow.
Journal ArticleDOI
Brain free fatty acid levels in rats sacrificed by decapitation versus focused microwave irradiation
TL;DR: The increase in free fatty acid levels following decapitation was completely absent in rats sacrificed by the microwave irradiation, and this microwave technique could be a valuable tool in determining free fatty acids and other heat stable compounds in brain tissue.
Journal ArticleDOI
The endogenous biosynthesis of prostaglandins by brain tissue in vitro.
TL;DR: The biosynthetic capacity of brain tissue in vitro appears to be orders of magnitude more than that of normal brain in situ.
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Effects of ischemia and electroconvulsive shock on free fatty acid pool in the brain.
The effect of incomplete cerebral ischemia on prostaglandin levels in rat brain.
E Shohami,J Rosenthal,Lavy S +2 more