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Influence of antioxidants on SOD activity in bovine sperm.

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TLDR
It was found a highly significative correlation between malondialdehyde production and SOD activity, which could be used in bovine spermatozoa as a metabolic indicator of membrane integrity.
Abstract
SOD activity and susceptibility to peroxidation on spermatozoa from frozen and fresh bovine semen were determined either in presence or not of synthetic (BHA) or natural (vitamin E) antioxidants. In sperm suspensions incubated with vitamin E, SOD activity was higher than in the control samples and the ones treated with BHA. It was found a highly significative correlation between malondialdehyde production and SOD activity. SOD activity could be used in bovine spermatozoa as a metabolic indicator of membrane integrity.

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Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon.

TL;DR: Successful semen cryopreservation enhances the advantages of AI over natural breeding, as long-term storage facilitates semen transport over distances, permits the quarantine of semen, and enables extended use of superior germplasm, even after the sire's death.
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Current status of sperm cryopreservation: why isn't it better?

TL;DR: In this article, the authors reviewed aspects of sperm cryopreservation paralleled by events of capacitation and evaluated the possible roles of sperm membrane cholesterol, reactive oxygen species, and seminal plasma as mediators of sperm function.
Journal ArticleDOI

Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa.

TL;DR: Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state and the major factors that may influence the post-thaw viability and fertility of buffalo spermatozoa are examined in detail.
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Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen

TL;DR: There was a negative association between TBARS and motility, suggesting that lipid peroxidation affected sperm motility and the addition of 200 microg/mL alpha-tocopherol in the semen extender could be useful to preserve boar spermatozoa against the oxidative stress generated by cryopreservation.
Journal ArticleDOI

Effect of natural antioxidants on frozen bovine semen preservation

TL;DR: Natural antioxidants would seem to exert a protective effect on the membrane of the cryopreserved spermatozoon in samples from good-quality semen, as well as lower malondialdehyde production than in the controls.
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