Book ChapterDOI
Integration of Micro Fluidic Bio-chip Design and Automatic fluorescent Identification for Rapid Sperm Mobility Assessment
Li-Chern Pan,Fang Chi Hsu,Yun Ying Wu,Fan-Gang Tseng,Da Jen Yao,Yieh Loong Tsai,Jiann-Loung Hwang +6 more
- pp 332-335
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TLDR
The proposed method is cost effective and is possible to serve as a protocol for rapid sperm quantitative assessment and confirmed that live sperms were able to cross the laminar stream created by the device and reach the sorting chamber with corresponding range from non-motile sperm to debris, as well as from living to dead ones.Abstract:
Since male abnormality has now been reported to account at least thirty percent of couples with fertility problem. Therefore, distinguishing and classification of sperm has become more and more important, especially when Assisted Reproductive Technology (ART) like in vitro fertilization (IVF) is involved. However, traditional sperm sorting methods all requires manual selection, which is tedious and often cause sperm damage during the screen process. Although, new design from our recent study, seemed to point for a possibility to make these kind of MISS device capable of separating sperms into various mobility sub-class. In addition, the Hoechst 33258 and propidium iodide double staining results, also confirmed that the most motile and health sperm is indeed able to leave the laminar stream and being sorted out at outlet-h, with least dead sperm or debris. Meanwhile, we observed the dead sperm had been dominate from clear red stains and live sperm are not as active as is corresponded with more diffused blue stains. In order to confirm the real sorting situation, we counted the amount of sperm in collected sample by flow cytometry and tried to establish the statistic model. Though the flow cytometry, we could detect the sperm fluorescent distribution and count the size of sperm population. Nevertheless, t-test with 95% confidence level also shown significant difference in live/dead sperm ratio. Therefore, from the combined result among flow cytometry, fluorescent stain, and the statistic model test, it is confirmed that live sperms were able to cross the laminar stream created by the device and reach the sorting chamber with corresponding range from non-motile sperms to debris, as well as from living to dead ones. It is concluded that the proposed method is cost effective and is possible to serve as a protocol for rapid sperm quantitative assessment.read more
References
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Journal ArticleDOI
Viability assessment of mammalian sperm using SYBR-14 and propidium iodide.
D. L. Garner,Larry Johnson +1 more
TL;DR: The proportion of living sperm in semen from six representative mammals was assessed by means of a dual staining technique using the stains SYBR-14 and propidium iodide (PI), a newly developed fluorescent nucleic acid stain that stained the nuclei ofliving sperm bright green as determined by simultaneous examination of fluorescence and motility.
Journal ArticleDOI
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Ralf Henkel,Wolf-Bernhard Schill +1 more
TL;DR: Sperm separation methods that yield a higher number of motile spermatozoa are glass wool filtration or density gradient centrifugation with different media and caffeine, pentoxifylline and 2-deoxyadenosine are substances that were used to stimulate motility.
Journal ArticleDOI
Passively driven integrated microfluidic system for separation of motile sperm
TL;DR: A self-contained integrated microfluidic system that can separate motile sperm from small samples that are difficult to handle using conventional sperm-sorting techniques, and opens the way for convenient bioassays based on sperm motility including at-home motiles sperm tests.
Journal ArticleDOI
Treatment of Male Infertility
TL;DR: There is a male factor in infertility in half of the couples, and even after a thorough evaluation, the cause of a man's lack of normal fertility usually remains unknown.
Proceedings ArticleDOI
A microfluidic device for separating motile sperm from nonmotile sperm via inter-streamline crossings
TL;DR: A microfluidic system with embedded reservoirs that sorts sperm based on the ability of motile sperm to move out of their initial streamlines in a laminar fluid stream that pumps fluids by hydrostatic pressure and surface tension is described.