Interaction of Proteins of Epididymal Origin with Spermatozoa
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Exposure of corpus and caudal spermatozoa to solutions containing 0.2 to 0.25 M salt reduced the amount of bound SEP to the level found in caput sperm whereas thistreatment had no effect on SEP bound to caput semen.Abstract:
The present work was devoted to the study of certain aspects of the interaction of rat specific epididymal proteins (SEP) with spermatozoa. The amounts of SEP bound to ratsperm from severalregionsof the epididymis were quantified using a micro-complement fixation technique. Sperm from the caput, corpus, and cauda epididymidis contained increasing amounts of both total SEP and of protein D-E on theirsurface.Incubations,eitherinutero or invitroinconditions similarto those known to favorcapacitation,resulted in a substantial loss of SEP from spermatozoa. Exposure of corpus and caudal spermatozoa to solutions containing 0.2 to 0.25 M salt reduced the amount of bound SEP to the levelfound in caput sperm whereas thistreatment had no effecton SEP bound to caput spermatozoa.read more
Citations
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Book ChapterDOI
Mechanisms of Fertilization in Mammals
TL;DR: This chapter will discuss how mammalian spermatozoa prepare themselves for fertilization and how the spermatozosa and eggs interact during fertilization.
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Epididymal epithelium: Its contribution to the formation of a luminal fluid microenvironment
TL;DR: This review focusses on current understanding of the contributions of the epididymal epithelium to the formation of a specialized luminal fluid microenvironment.
Book ChapterDOI
Molecules involved in mammalian sperm-egg interaction.
TL;DR: These molecules and the processes involved in gamete interaction are reviewed in this chapter within a physiological context.
Journal ArticleDOI
Redistribution of a rat sperm epididymal glycoprotein after in vitro and in vivo capacitation
TL;DR: Results indicate that epididymal protein DE migrates to the equatorial segment under in vitro and in vivo capacitating conditions and suggest a possible association between the redistribution of DE and the occurrence of the AR.
Book ChapterDOI
Epididymal Cell Types and Their Functions
Louis Hermo,Bernard Robaire +1 more
TL;DR: The mammalian epididymis is a highly coiled duct that links the efferent ducts to the vas deferens and plays an important role in sperm transport, concentration, protection, and storage.
References
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Journal ArticleDOI
The structure and fuction of the Epididymis. 1. The histology of the Rat Epididymis.
TL;DR: The epididymis possesses an epithelium containing six cell types-principal, basal, ciliated, apical, halo, and clear cells-each of which is described, which permits two zones to be recognized in the efferent ducts.
Journal ArticleDOI
Studies on the function of the epididymis.
Journal ArticleDOI
Purification and Localization of Acidic Epididymal Glycoprotein (AEG): A Sperm Coating Protein Secreted by the Rat Epididymis
TL;DR: Localization of AEG using an immunoperoxidase method revealed that it is secreted largely by the epithelium of caput and corpus beginning with the region distal to the initial segment, and appears to be secreted by a specific cell type, probably the so-called “principal” cell.
Journal ArticleDOI
Androgen-controlled specific proteins in rat epididymis.
TL;DR: Preliminary evidence indicates that the proteins in bands C, D and E can be removed from caput spermatozoa by washing, and was found to be sensitive to changes in androgen in the blood.
Journal ArticleDOI
The distribution of negative surface charges on mammalian spermatozoa.
Ryuzo Yanagimachi,Ryuzo Yanagimachi,Y. D. Noda,Y. D. Noda,M. Fujimoto,M. Fujimoto,Garth L. Nicolson,Garth L. Nicolson +7 more
TL;DR: Comparison of testicular, caput and cauda epididymal spermatozoa of rabbit, bull and hamster suggests that the negative surface charges of spermutozoa increase with epididcyal maturation of spermatoza, indicating that sperm tails have more acidic negative charges than heads.