scispace - formally typeset
Open AccessJournal ArticleDOI

Kinetic analysis of the hydrolysis of lecithin monolayers by phospholipase A.

George Zografi, +2 more
- 01 Dec 1971 - 
- Vol. 7, Iss: 4, pp 185-206
TLDR
Comparison of the monolayer and bulk methods showed that both techniques are complementary, and the pH dependence and calcium requirements of the enzymatic reaction are similar under monolayers and bulk conditions.
About: 
This article is published in Chemistry and Physics of Lipids.The article was published on 1971-12-01 and is currently open access. It has received 115 citations till now. The article focuses on the topics: Monolayer & Substrate (chemistry).

read more

Citations
More filters
Journal ArticleDOI

Biomolecular interactions at phospholipid-decorated surfaces of liquid crystals

Jeffrey M. Brake, +3 more
- 19 Dec 2003 - 
TL;DR: These results provide principles for label-free monitoring of aqueous streams for molecular and biomolecular species without the need for complex instrumentation.
Journal ArticleDOI

Action of phospholipase A at interfaces.

Robert Verger, +2 more
- 10 Jun 1973 - 
TL;DR: A quantitative comparison between enzyme kinetics obtained by monolayer and bulk techniques is attempted using a common definition of the "quality" of the lipid-water interface, and the concept of a penetration site on the enzyme is discussed.
Book ChapterDOI

Structure and function of phospholiphase A2

Hubertus M. Verheij, +2 more
- 01 Jan 1981 - 
Journal ArticleDOI

Methods for lipase detection and assay: a critical review

Frédéric Beisson, +3 more
- 01 Feb 2000 - 
Journal ArticleDOI

Enzyme reactions in a membrane model 1: A new technique to study enzyme reactions in monolayers

Robert Verger, +1 more
- 01 Feb 1973 - 
TL;DR: Enzymic hydrolysis by pancreatic phospholipase A of 3-sn-didodecanoyl phosphatidylglycerol was studied under constant surface pressure and the recorded kinetic plots are linear, in contrast to the non-linear kinetic plots obtained with the usual one-compartment trough.
References
More filters
Journal ArticleDOI

The colorimetric determination of phosphorus

Cyrus H. Fiske, +1 more
- 01 Dec 1925 - 
Journal ArticleDOI

Action de la lipase pancréatique sur les esters en émulsion

L. Sarda, +1 more
- 01 Dec 1958 - 
TL;DR: De considerer la lipase pancreatique comme une esterase particuliere destinee a agir sur des esters insolubles au niveau of l'interface separant ces esters de l'eau, un calcul fait intervenir the “concentration molaire interfaciale” du substrat.
Journal ArticleDOI

Monolayer characteristics of saturated 1,2-diacyl phosphatidylcholines (lecithins) and phosphatidylethanolamines at the air-water interface

M.C. Phillips, +1 more
- 05 Nov 1968 - 
TL;DR: The lecithins formed more expanded films than the phosphatidylethanolamines and this is interpreted in terms of differences in the size and orientation of the polar groups and this transition occuring in the bimolecular lamellae in water corresponds to the transition from condensed to expanded monolayer.
Journal ArticleDOI

The substrate specificity of phospholipase a.

L.L.M. Van Deenen, +1 more
- 01 Jan 1963 - 
TL;DR: Investigations on variously modified analogues of phospholipids elucidated the following substrate characteristics forospholipase A (Crotalus adamanteus): l-α-Lecithins containing fatty acids with greatly varying chain length are susceptible to phospholIPase A action; however, certain water-soluble short-chain compounds are hydrolysed at a very slow rate only.
Journal ArticleDOI

Synthesis of lecithins by acylation of O-(sn-glycero-3-phosphoryl) choline with fatty acid anhydrides

E.Cubero Robles, +1 more
- 17 Dec 1969 - 
TL;DR: A new method has been developed for the partial synthesis of saturated, unsaturated and mixed-acid lecithins using the free O-(sn-glycero-3-phosphoryl) choline and its CdCl2 complex as starting materials.