Journal ArticleDOI
Leukocyte Oxidase: Defective Activity in Chronic Granulomatous Disease
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TLDR
The addition of nitroblue tetrazolium to a leukocyte suspension appears to provide a sensitive diagnostic screening test for chronic granulomatous disease.Abstract:
The intact leukocytes of two children with chronic granulomatous disease fail to reduce nitroblue tetrazolium during phagocytosis. This is due to defective operation of an oxidase of reduced nicotinamide adenine dinucleotide that is insensitive to cyanide and that indirectly stimulates the oxidation of glucose-6-phosphate in leukocytes. Such leukocytes undergo no increase in oxygen consumption or in activity of the hexose monophosphate shunt during phagocytosis, although lactate production is normal. The addition of nitroblue tetrazolium to a leukocyte suspension appears to provide a sensitive diagnostic screening test for this disease.read more
Citations
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The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology
Karen Bedard,Karl-Heinz Krause +1 more
TL;DR: This review summarizes the current state of knowledge of the functions of NOX enzymes in physiology and pathology.
Journal ArticleDOI
Oxygen-dependent microbial killing by phagocytes (second of two parts).
TL;DR: Since Metschnikoff's discovery, hundreds of scientists studying dozens of species have reported thousands of studies on these cells, perhaps the most widely recognized of which are those of the eminent English scientists.
Journal ArticleDOI
Quantitative Nitroblue Tetrazolium Test in Chronic Granulomatous Disease
TL;DR: Chronic granulomatous disease is an X-linked defect in the killing of certain bacteria by peripheral blood granulocytes and may be detected with the nitroblue tetrazolium (NBT) test.
Journal ArticleDOI
Genetic, biochemical, and clinical features of chronic granulomatous disease
TL;DR: The reduced nicotinamide dinucleotide phosphate (NADPH) oxidase complex allows phagocytes to rapidly convert O2 to superoxide anion which then generates other antimicrobial reactive oxygen intermediates, such as H2O2, hydroxyl anion, and peroxynitrite anion.
Journal ArticleDOI
INFECTION AND NITROBLUE-TETRAZOLIUM REDUCTION BY NEUTROPHILS: A Diagnostic Aid
TL;DR: Venous blood from healthy volunteers, patients with known bacterial disease, and patients with non-bacterial illnesses was incubated with a solution of nitroblue-tetrazolium (N.B.T.).
References
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Journal ArticleDOI
Fatal granulomatous disease of childhood. An inborn abnormality of phagocytic function.
Journal Article
A fatal granulomatosus of childhood: the clinical study of a new syndrome.
Berendes H,Bridges Ra,Good Ra +2 more
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Enzymatic basis of the respiratory stimulation during phagocytosis.
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Thirteen boys with progressive septic granulomatosis
TL;DR: Thirteen boys are described who present a syndrome of chronic suppurative lymphadenitis, chronic dermatitis, Chronic pulmonary disease, and hepatosplenomegaly, with an eventually fatal outcome, with the existence of an as yet unidentified physiological defect suggested.