Liquid Chromatography of Adenine, Caffeine, Theobromine, Theophylline, Tannic Acid, Xanthine and its Application to Tea Analysis

TLDR: In this article, a polystyrenesulfonic acid resin in the hydrogen form is used for chromatographic separation of adenine, caffeine, theobromine, theophylline, tannic acid, xanthine and tea components with aqueous ethanol as eluent.

Abstract: A polystyrenesulfonic acid resin in the hydrogen form is a useful stationary phase for chromatographic separation of adenine, caffeine, theobromine, theophylline, tannic acid, xanthine and tea components with aqueous ethanol as eluent. The results indicated that caffeine, theophylline, theobromine, adenine, xanthine and tannic acid could be separated on a column of Dowex-50W-X8(H)+ by elution with 25% ethanol as eluent.