Journal ArticleDOI
Low level laser therapy modulates viability, alkaline phosphatase and matrix metalloproteinase-2 activities of osteoblasts
Flávia Amadeu de Oliveira,Adriana Arruda Matos,Sandra Satiko Matsuda,Marília Afonso Rabelo Buzalaf,V. S. Bagnato,Maria Aparecida de Andrade Moreira Machado,Carla Andreotti Damante,Rodrigo Cardoso de Oliveira,Camila Peres-Buzalaf +8 more
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TLDR
When used at these specific parameters, LLL modulates both preosteoblast viability and differentiation highlighted by the increased ALP and MMP-2 activities induced by irradiation.Abstract:
Low level laser therapy (LLLT) has been shown to stimulate bone cell metabolism but their impact on the matrix metalloproteinase (MMP) expression and activity is little explored. This study evaluated the influence of LLLT at two different wavelengths, red and infrared, on MC3T3-E1 preosteoblast viability, alkaline phosphatase (ALP) and MMP-2 and -9 activities. To accomplish this, MC3T3-E1 cells were irradiated with a punctual application of either red (660 nm; InGaAIP active medium) or infrared (780 nm; GaAlAs active medium) lasers both at a potency of 20 mW, energy dose of 0.08 or 0.16 J, and energy density of 1.9 J/cm2 or 3.8 J/cm2, respectively. The control group received no irradiation. Cellular viability, ALP and MMP-2 and -9 activities were assessed by MTT assay, enzymatic activity and zymography, respectively, at 24, 48 and 72 h. The treatment of cells with both red and infrared lasers significantly increased the cellular viability compared to the non-irradiated control group at 24 and 48 h. The ALP activity was also up modulated in infrared groups at 24 and 72 h, depending on the energy densities. In addition, the irradiation with red laser at the energy density of 1.9 J/cm2 promoted an enhancement of MMP-2 activity at 48 and 72 h. However, no differences were observed for the MMP-9 activity. In conclusion, when used at these specific parameters, LLL modulates both preosteoblast viability and differentiation highlighted by the increased ALP and MMP-2 activities induced by irradiation.read more
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Low-level laser therapy induces human umbilical vascular endothelial cell proliferation, migration and tube formation through activating the PI3K/Akt signaling pathway.
Yue Li,Qihua Xu,Menglin Shi,Puying Gan,Qin Huang,Anan Wang,Guiying Tan,Yangbin Fang,Hongfei Liao +8 more
TL;DR: It is suggested that the inhibition of the PI3K/Akt signaling pathway attenuated the effects of LLLT on proliferation, migration, and angiogenesis of HUVECs.
Journal ArticleDOI
Low power laser irradiation and human adipose-derived stem cell treatments promote bone regeneration in critical-sized calvarial defects in rats
Yan-Hsiung Wang,Jyun Yi Wu,Su Chii Kong,Min Hsuan Chiang,Mei-Ling Ho,Ming Long Yeh,Chia-Hsin Chen +6 more
TL;DR: ADSC and LPLI treatments improved fracture repair in critical-sized calvarial defects in rats and further enhances the bone healing process, and PLGA had no cytotoxic effect on human ADSCs.
Journal ArticleDOI
Physicochemical bisphosphonate immobilization on titanium dioxide thin films surface by UV radiation for bio-application
Erika S. Bronze-Uhle,Leonardo F.G. Dias,Luciana D. Trino,Adriana Arruda Matos,Rodrigo Cardoso de Oliveira,Paulo Noronha Lisboa-Filho +5 more
TL;DR: In this paper, the physicochemical properties of bisphosphonates (BP) were evaluated on the titanium surface containing hydroxylated TiO2 film by UV radiation and the results indicated that the UV treatment increased the surface wettability and allowed an oriented immobilization of BP.
Journal ArticleDOI
Physicochemical characterization of albumin immobilized on different TiO2 surfaces for use in implant materials
Erika S. Bronze-Uhle,Leonardo F.G. Dias,Luciana D. Trino,Adriana Arruda Matos,Rodrigo Cardoso de Oliveira,Paulo Noronha Lisboa-Filho +5 more
TL;DR: TiO2-APTMS substrate with -NH2 groups on the surface more efficiently interacts with components of the extracellular matrix, presenting high cell viability at 48 h and better viability and mineralization results.
Journal ArticleDOI
In Vitro Cytological Responses against Laser Photobiomodulation for Periodontal Regeneration.
Yujin Ohsugi,Hiromi Niimi,Tsuyoshi Shimohira,Masahiro Hatasa,Sayaka Katagiri,Akira Aoki,Takanori Iwata +6 more
TL;DR: Light is shed on basic research that would ultimately lead to clinical application of periodontal phototherapy in the future through this review of the molecular biological effects of diode, Nd:YAG, Er:Y AG, Er,Cr:YSGG, and CO2 lasers irradiation on cells inperiodontal tissues.
References
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Journal ArticleDOI
Structure and function of matrix metalloproteinases and TIMPs
TL;DR: The members of the MMP family are introduced and their domain structure and function, proenyme activation, the mechanism of inhibition by TIMPs and their significance in physiology and pathology are discussed.
Journal ArticleDOI
Quantitative zymography: detection of picogram quantities of gelatinases.
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Journal ArticleDOI
The role of gelatinases in colorectal cancer progression and metastasis.
TL;DR: It is concluded that the use of MMP inhibitors to treat cancer should be considered carefully because of the multifunctionality of gelatinases, it is unpredictable at what stage of cancer development and in which processes gelatinase activity is involved.
Journal ArticleDOI
Molecular mechanisms of cell proliferation induced by low power laser irradiation.
Xuejuan Gao,Da Xing +1 more
TL;DR: Hepatocyte growth factor receptor (c-Met), a member of tyrosine protein kinase receptors (TPKR), is phosphorylated during LPLI-induced proliferation, but tumor necrosis factor alpha (TNF-alpha) receptor has not been affected.
Journal ArticleDOI
Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence.
Luciana Almeida-Lopes,Josepa Rigau,Renato Amaro Zângaro,João Guidugli‐Neto,Márcia Martins Marques Jaeger +4 more
TL;DR: A primary culture of human gingival fibroblasts is developed in order to analyse the effect of LLLT on the in vitro proliferation of gingivals and to improve wound healing.