Microsporogenesis and microgametogenesis of Cardiospermum grandiflorum and Urvillea chacoensis (Sapindaceae, Paullinieae)

TLDR: It can be concluded that male sterility is characterised by failure to produce functional pollen grains, an event that would be associated with the persistence of tapetal cells.

Abstract: Microsporogenesis and microgametogenesis of two species, Cardiospermum grandiflorum Sw. and Urvillea chacoensis Hunz. (Sapindaceae, Paullinieae), were studied using light and transmission electron microscopy. Both species are monoecious, with staminate and hermaphrodite, although functionally pistillate, flowers. A comparative pollen-development study of these two floral morphs is reported. For the present study, five stages of pollen ontogeny were identified. The development of the anther wall is of basic type. Its wall consists of epidermis, endothecium, two middle layers and a uninucleate secretory tapetum. The microspore tetrads are tetrahedral. The mature anther in staminate flowers presents the endothecium with well developed fibrillar thickenings, remains of tapetal cells, a single locule formed in the theca by dissolution of the septum before anther dehiscence and two-celled pollen grains when shed. In functionally pistillate flowers, the mature anthers present remnants of the middle layers, tapetal cells without signs of degradation, the theca with two locules and pollen grains uni- or bicellular, some of them with the cytoplasm collapsed. These anthers are not dehiscent. It can be concluded that male sterility is characterised by failure to produce functional pollen grains, an event that would be associated with the persistence of tapetal cells. Ultrastructural analysis clearly shows the difference in tapetal cells between the two flower morphs.