Multidrug-Resistant Methicillin-Resistant Coagulase-Negative Staphylococci in Healthy Poultry Slaughtered for Human Consumption
Vanessa Silva,Manuela Caniça,Eugénia Ferreira,Madalena Vieira-Pinto,C. Saraiva,José Eduardo Pereira,José Luis Capelo,Gilberto Igrejas,Patrícia Poeta +8 more
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TLDR
There was a frequent occurrence of multidrug resistance in MRCoNS isolated from healthy poultry in Portugal, and most isolates showed resistance to erythromycin, clindamycin, penicillin, tetracycline, ciprofloxacin and fusidic acid and harbored the ermB, ermC, mphC, tetK, tetL, tetM and tetO genes.Abstract:
Coagulase-negative staphylococci are commensals that are known to be prevalent in most environments, and they are also an important reservoir of antimicrobial-resistant genes. Staphylococcal infections in animal husbandry are a high economic burden. Thus, we aimed to determine the prevalence and species diversity of methicillin-resistant coagulase-negative staphylococci (MRCoNS) in poultry slaughtered for human consumption and to study the antimicrobial resistance of the isolates. Swab samples were recovered from 220 commercial chickens, homebred chickens and quails. Species identification was performed using MALDI-TOF. Antimicrobial susceptibility testing was performed by the disc diffusion method against 14 antimicrobials. The presence of antimicrobial-resistant genes was investigated by polymerase chain reaction. Totals of 11 (19.6%), 13 (20.3%), and 51 (51%) MRCoNS were isolated from commercial chickens, homebred chickens and quails, respectively. S. lentus was isolated from all homebred chickens, whereas 11 S. lentus and 2 S. urealyticus were isolated from commercial chickens. As for quails, the most prevalent MRCoNS were S. urealyticus. Almost all isolates had a multidrug-resistant profile and carried the mecA gene. Most isolates showed resistance to erythromycin, clindamycin, penicillin, tetracycline, ciprofloxacin and fusidic acid and harbored the ermA, ermB, ermC, mphC tetK, tetL, tetM and tetO genes. This study showed a frequent occurrence of multidrug resistance in MRCoNS isolated from healthy poultry in Portugal.read more
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Occurrence and Characteristics of Staphylococcus aureus Strains along the Production Chain of Raw Milk Cheeses in Poland
TL;DR: In this paper , the authors investigated the prevalence of S. aureus in the artisanal cheese production chain from unpasteurized milk and characterized them using microbroth dilution and biofilm formation by in vitro assay.
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Biofilm Formation of Staphylococcus aureus from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance
Vanessa Silva,Elisete Correia,José Eduardo Pereira,Camino González-Machado,Rosa Capita,Carlos Alonso-Calleja,Gilberto Igrejas,Patrícia Poeta +7 more
TL;DR: The results provide important information on theBiofilm-forming capacity of animal-adapted S. aureus isolates, which may have potential implications for the development of new biofilm-targeted therapeutics.
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Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
Vanessa Silva,Elisete Correia,José Eduardo Pereira,Camino González-Machado,Rosa Capita,Carlos Alonso-Calleja,Gilberto Igrejas,Patrícia Poeta +7 more
TL;DR: Evaluated the biofilm-forming capacity of CoNS and S. pseudintermedius isolated from several animal species and the effect of conventional antimicrobials on biofilm reduction and a positive association between biofilm formation and multidrug resistance as well as resistance to individual antimicrobialbials.
Journal ArticleDOI
Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus from Hunters and Hunting Dogs
Vanessa Silva,Manuela Caniça,Vera Manageiro,Madalena Vieira-Pinto,José Eduardo Pereira,Luís Maltez,Patrícia Poeta,Gilberto Igrejas +7 more
TL;DR: A moderate colonization of S. aureus in hunting dogs and their owners was detected in this study and a few dog- to-dog and dog-to-human possible transmissions were identified.
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Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings: A Cross-Sectional Study from the One Health Perspective
Vanessa Silva,Susana Correia,Jaqueline Rocha,Célia M. Manaia,Adriana Silva,Juan García-Díez,José Eduardo Pereira,Teresa Semedo-Lemsaddek,Gilberto Igrejas,Patrícia Poeta +9 more
TL;DR: Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers, which may suggest a possible transmission.
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