Observation's on the germination of Endogone spores

TLDR: It was possible to obtain greater than 90% yields of germinated E. mosseae Nicolson & Gerdemann spores on distilled water agar with improved germination of some spore sources by the addition to the agar of nicotinic acid or thiamine HCl.

Abstract: Although reliable germination of Endogone spores has previously been difficult to achieve, it was possible to obtain greater than 90% yields of germinated E. mosseae Nicolson & Gerdemann spores on distilled water agar. Zinc and manganese, at the concentrations present in many commercially available agars could completely inhibit spore germination. Spores from freshly harvested sporocarps germinated slowly and this was improved by storing the sporocarps at 6° for several weeks. The germination of some spore sources was improved by the addition to the agar of nicotinic acid or thiamine HCl.