PCR amplification and comparison of nucleotide sequences from the groESL heat shock operon of Ehrlichia species.
Reads0
Chats0
TLDR
Degenerate PCR primers derived from conserved regions of the eubacterial groESL heat shock operon were used to amplifyGroESL sequences of Ehrlichia equi, EHRlichia phagocytophila, and the agent of human granulocytic ehrlichiosis, and a phylogenetic tree derived from deduced GroEL amino acid sequences was similar to trees based on 16S rRNA gene sequences.Abstract:
Degenerate PCR primers derived from conserved regions of the eubacterial groESL heat shock operon were used to amplify groESL sequences of Ehrlichia equi, Ehrlichia phagocytophila, the agent of human granulocytic ehrlichiosis (HGE), Ehrlichia canis, Bartonella henselae, and Rickettsia rickettsii. The groESL nucleotide sequences were less conserved than the previously determined 16S rRNA gene sequences of these bacteria. A phylogenetic tree derived from deduced GroEL amino acid sequences was similar to trees based on 16S rRNA gene sequences. Nucleotide sequences obtained from clinical samples containing E. equi, E. phagocytophila, or the HGE agent were very similar (99.9 to 99.0% identity), and the deduced amino acid sequences were identical. Some divergence was evident between nucleotide sequences amplified from samples originating from the United States (E. equi and the HGE agent) and sequences from the European species, E. phagocytophila. A single pair of PCR primers derived from these sequences was used to detect E. chaffeensis and HGE agent DNA in blood samples from human patients with ehrlichiosis.read more
Citations
More filters
Journal ArticleDOI
Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila.
J. S. Dumler,Anthony F. Barbet,C. P.J. Bekker,G. A. Dasch,G. H. Palmer,Stuart C. Ray,Y. Rikihisa,F. R. Rurangirwa +7 more
TL;DR: It is proposed that all members of the tribes Ehrlichieae and Wolbachieae be transferred to the family Anaplasmataceae and that the tribe structure of the family Rickettsiaceae be eliminated.
Journal ArticleDOI
Ehrlichia chaffeensis: a Prototypical Emerging Pathogen
TL;DR: The current understanding of the microbiology, pathogenesis, and clinical manifestations associated with this pathogen are summarized but focus primarily on discussing various ecological factors responsible for the recent recognition of this important and potentially life-threatening tick-borne disease.
Journal ArticleDOI
Guidelines for the diagnosis of tick-borne bacterial diseases in Europe
Philippe Brouqui,Fátima Bacellar,G. Baranton,Richard J. Birtles,A. Bjoërsdorff,José Ramón Blanco,Giuseppe Caruso,Marina Cinco,Pierre-Edouard Fournier,E. Francavilla,Mogens Jensenius,J. Kazar,Hermann Laferl,András Lakos,S. Lotric Furlan,Max Maurin,José A. Oteo,Philippe Parola,C. Perez-Eid,O. Peter,D. Postic,Didier Raoult,A Téllez,Yiannis Tselentis,B. Wilske +24 more
TL;DR: These guidelines aim to help clinicians and microbiologists in diagnosing infection transmitted by tick bites and to provide the scientific and medical community with a better understanding of these infectious diseases.
Journal ArticleDOI
Human Ehrlichiosis and Anaplasmosis
TL;DR: Recent advances in the understanding of ehrlichial diseases related to microbiology, epidemiology, diagnosis, pathogenesis, immunity, and treatment of the 2 prevalent tick-borne diseases found in the United States are reviewed.
Journal ArticleDOI
Novel genetic variants of Anaplasma phagocytophilum, Anaplasma bovis, Anaplasma centrale, and a novel Ehrlichia sp. in wild deer and ticks on two major islands in Japan.
Makoto Kawahara,Yasuko Rikihisa,Quan Lin,Emiko Isogai,Kenji Tahara,Asao Itagaki,Yoshimichi Hiramitsu,Tomoko Tajima +7 more
TL;DR: The results suggest that enzootic cycles of Ehrlichia and Anaplasma species distinct from those found in the United States or Europe have been established in wild deer and ticks in Japan.
References
More filters
Journal ArticleDOI
Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes.
TL;DR: DNA sequences from specific genes, amplified by the polymerase chain reaction technique, were used as substrata for nonisotopic restriction endonuclease fragment length polymorphism differentiation of rickettsial species and genotypes, and the estimated relationships deduced from these genotypic data correlate reasonably well with established ricksettsial taxonomic schemes.
Journal ArticleDOI
Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease
TL;DR: Six patients from northern Minnesota and Wisconsin with a febrile illness accompanied by granulocytic cytoplasmic morulae suggestive of ehrlichial infection were identified and were shown to be infected by an Ehrlichia species never previously reported to infect humans.
Journal ArticleDOI
Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction.
TL;DR: This method should be useful for increasing the amounts of bacterial 16S ribosomal DNA sequences for the purposes of sequencing and probing, and should have a broad range of applications, including the detection and identification of known pathogens that are difficult to culture.
Journal ArticleDOI
Ehrlichia chaffeensis, a new species associated with human ehrlichiosis.
TL;DR: The sequence comparisons indicate that the human ehrlichiosis agent is a new species most closely related to E. canis and more distantly related to other Ehrlichia spp, and it is proposed that this species be named EHRlichia chaffeensis sp.
Journal ArticleDOI
Human granulocytic ehrlichiosis in the upper Midwest United States. A new species emerging
Johan S. Bakken,J. Stephen Dumler,Sheng Min Chen,Mark R. Eckman,Linda Van Etta,David H. Walker +5 more
TL;DR: All 12 patients have been infected with a granulocytic Ehrlichia species, reflecting a recently described new disease entity, and early detection and treatment with tetracycline drugs appear to offer the best chance for complete recovery.