Journal ArticleDOI
Phosphotungstic Acid-Chromic Acid as a Selective Electron-Dense Stain for Plasma Membranes of Plant Cells
TLDR
A mixture consisting of 1% phosphotungstic acid in 10% chromic acid selectively stains the plasma membrane of plant cells, retained in homogenates and serves to identify the plant plasma membrane in cell fractions.Abstract:
A mixture consisting of 1% phosphotungstic acid (PTA) in 10% chromic acid (CrO3) selectively stains the plasma membrane of plant cells. Whole tissue or pelleted cell fractions are prepared for electron microscopy using conventional methods including glutaraldehyde fixation and OsO4 postfixation, dehydration in acetone and embedding in Epon. To stain the plasma membrane, thin sections are transferred with a plastic loop to the surface of a 1% aqueous solution of periodic acid for 30 min for destaining. Following transfer through 5 distilled water rinses, the sections are exposed to the PTA-CrO3 mixture for 5 min, rinsed and mounted on grids for viewing with the electron microscope. The selectivity of the stain is retained in homogenates and serves to identify the plant plasma membrane in cell fractions.read more
Citations
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Book ChapterDOI
The Structure and Properties of the Cell Surface Coat
TL;DR: This chapter discusses the structure and properties of thecell surface coat, a relatively strong, hydrophilic coat which is a permanent feature of the cell surface complex.
Book ChapterDOI
Plasma membrane ATPase of fungi and plants as a novel type of proton pump
TL;DR: Evidence obtained at different levels of biological organization is presented that characterizes the plasma membrane ATPase of fungi and plants as a novel type of proton pump.
Journal ArticleDOI
Rhodophycean pit plugs an ultrastructural survey with taxonomic implications
TL;DR: The pit plug character confirms the validity of the order Palmariaes and indicates that the Nemaliales and Cryptonemiales are heterogeneous assemblages and Revision of these groups along lines indicated by the plug character is supported by significant features of morphology and reproduction.
Journal ArticleDOI
Partition of membrane particles in aqueous two-polymer phase system and its practical use for purification of plasma membranes from plants.
TL;DR: A simplified method for the isolation of a plasma membrane-enriched fraction from plants utilizing an aqueous two-polymer phase system is outlined and may be applicable to a wide variety of plants and plant tissue including green leaves.
Book ChapterDOI
A Critical Evaluation of Markers Used in Plasma Membrane Purification
S. Widell,C. Larsson +1 more
TL;DR: A membrane marker is any property that can be used to identify a specific membrane, which is not only confined to a single membrane, but is also uniformly distributed in the membrane, thus constituting an absolute marker for that membrane.
References
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Journal ArticleDOI
THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY
TL;DR: The stain reported here differs from previous alkaline lead stains in that the chelating agent, citrate, is in sufficient excess to sequester all lead present, and is less likely to contaminate sections.
Journal ArticleDOI
A low-viscosity epoxy resin embedding medium for electron microscopy.
TL;DR: A low-viscosity embedding medium based on ERL-4206 is recommended for use in electron microscopy and has a long pot life of several days and infiltrates readily because of its low viscosity.
Journal ArticleDOI
Improvements in epoxy resin embedding methods.
TL;DR: Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenientembedding methods for electron microscopy.
Journal ArticleDOI
Studies on plasma membranes. i. chemical composition and enzyme content of plasma membranes isolated from rat liver
TL;DR: The possibility that the plasma-membrane preparations were contaminated by microsomal elements is discussed, and, on account of the available evidence, the conclusion is reached that there is no reason to assume that this was the case.