Patent
Probe method and composition for detecting different dna sequences
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TLDR
In this article, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target poynucleotide.Abstract:
Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In practicing the method, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive elctrophoretic mobility in a sieving matrix, to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.read more
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Patent
Coupled amplification and ligation method
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Maarten Holkers,Jim Swildens +1 more
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Patent
Uncharged morpholino-based polymers having achiral intersubunit linkages
TL;DR: In this article, a polymer composition is disclosed composed of morpholino subunit structures which are linked together by uncharged, achiral linkages, which are one to three atoms in length, joining the morpholine nitrogen of one subunit to the 5' exocyclic carbon of an adjacent subunit.
Patent
A thermostable ligase mediated dna amplification system for the detection of genetic diseases
TL;DR: In this article, the cloning of the gene of a thermophilic DNA ligase, from Thermus aquaticus strain HB8, was described, and the use of this ligase for the detection of specific sequences of nucleotides in a variety of nucleic acid samples.
Patent
Detecting agent carrying polymer having multiple units of visualization monomer.
TL;DR: In this paper, the association between a detecting agent such as an antibody or complementary nucleotide sequence and its target molecule is visualized using diagnostic monomers such as enzymes, polypeptides, tagged polyols, tagged olyolefins and tagged carbohydrates.