Patent
Coupled amplification and ligation method
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TLDR
A method based on polymerase chain reaction (PCR) amplification and oligonucleotide ligase assay (OLA) reaction is provided for analyzing complex genetic systems in a single reaction vessel as mentioned in this paper.Abstract:
A method based on polymerase chain reaction (PCR) amplification and oligonucleotide ligase assay (OLA) reaction is provided for analyzing complex genetic systems in a single reaction vessel The method involves simultaneously incubating a sample containing one or more target polynucleotides with PCR primers and OLA probes in a single reaction mixture The presence of variant polynucleotide sequences in the sample is determined by detecting and identifying the products of the OLA reactionread more
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Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
TL;DR: In this paper, the use of a ligase detection reaction coupled to a polymerase chain reaction has been proposed for the detection of nucleic acid sequence differences using coupled ligases detection reaction and polymerases chain reaction.
Patent
Detection of nucleic and sequence differences using the ligase detection reaction with addressable arrays
TL;DR: In this paper, the authors describe a method for identifying one or more of a plurality of sequences differing by single base changes, insertions, deletions, or translocations in target nucleotide sequences.
Patent
Oligonucleotide compositions with enhanced efficiency
Tod M. Woolf,Margaret F Taylor +1 more
Abstract: The oligonucleotide compositions of the present invention make use of combinations of oligonucleotides. In one aspect, the invention features an oligonucleotide composition including at least 2 different oligonucleotides targeted to a target gene. This invention also provides methods of inhibiting protein synthesis in a cell and methods of identifying oligonucleotide compositions that inhibit synthesis of a protein in a cell.
Patent
Massively parallel signature sequencing by ligation of encoded adaptors
TL;DR: In this article, a method of nucleic acid sequence analysis based on the ligation of one or more sets of encoded adaptors to the terminus of a target polynucleotide was proposed.
Patent
Double-stranded oligonucleotides
Todd M. Woolf,Kristin Wiederholt +1 more
TL;DR: In this paper, duplex RNAi compositions, which possess improved properties over those taught in the prior art, are disclosed, and optimized antisense oligomer compositions and method for making and using the both in vitro systems and therapeutically.
References
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Patent
Process for amplifying nucleic acid sequences
TL;DR: In this article, a process for amplifying any desired specific nucleic acid sequence contained in a mixture of nucleic acids or mixture thereof is described, which can be repeated as often as desired.
Patent
Process for amplifying, detecting, and/or-cloning nucleic acid sequences
Kary Banks Mullis,Norman Arnheim,Randall K. Saiki,Henry A. Erlich,Glenn Thomas Horn,Stephen J. Scharf +5 more
TL;DR: In this paper, the authors proposed a method for synthesizing nucleic acid sequences using primers, which can be repeated stepwise or simultaneously and can be replicated as often as desired.
Journal ArticleDOI
A ligase-mediated gene detection technique
TL;DR: An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule, which permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.
Journal ArticleDOI
Genetic disease detection and DNA amplification using cloned thermostable ligase
TL;DR: This thermostable enzyme, DNA ligase, is harnessed in the assay reported here that both amplifies DNA and discriminates a single-base substitution, and was exploited to detect 200 target molecules as well as to discriminate between normal beta A- and sickle beta S- globin genotypes from 10-microliters blood samples.
Patent
Detection of specific sequences in nucleic acids
TL;DR: In this article, the authors proposed a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated and is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid.