Open AccessJournal Article
Research letterDetermination of DNA base composition by reversed-phase high-performance liquid chromatography
Jin Tamaoka,Kazuo Komagata +1 more
TLDR
The system described here gives a direct and precise method for determining DNA base composition and the relative standard error of nucleoside analysis was less than 1%.Abstract:
DNA base composition was determined by reversed-phase high-performance liquid chromatography (HPLC). DNA was hydrolysed into nucleosides with nuclease P1 and bacterial alkaline phosphatase. The mixture of nucleosides was applied to HPLC without any further purification. One determination by chromatography needed 2 μg of hydrolysed nucleosides and took only 8 min. The relative standard error of nucleoside analysis was less than 1%. The system described here gives a direct and precise method for determining DNA base composition.read more
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Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species
TL;DR: One hundred and nineteen strains of alkaliphilic and alkalitolerant, aerobic endospore-forming bacteria were examined for 47 physiological and biochemical characters, and DNA base composition, and nine of the remainder are proposed as new species.
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Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov.
TL;DR: The taxonomic positions of six strains of Chryseobacterium meningosepticum and C. miricola were re-evaluated by using a polyphasic taxonomic approach and phylogenetic and phenotypic data showed that they should be transferred to a new genus, Elizabethkingia gen. nov.
Journal ArticleDOI
Taxonomic revision of water-bloom-forming species of oscillatorioid cyanobacteria.
Shoichiro Suda,Makoto M. Watanabe,Shigeto Otsuka,Aparat Mahakahant,Wichien Yongmanitchai,Napavarn Nopartnaraporn,Yongding Liu,John G. Day +7 more
TL;DR: A polyphasic approach was used to clarify the taxonomy of the water-bloom-forming oscillatorioid cyanobacteria and proposed either emended or new taxonomic descriptions for Planktothrix agardhii, PlankTothrix rubescens, and Limnothrix redekei.
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Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa.
TL;DR: Four N(2)O-producing facultative aerobes, ED1(T), ED5 (T), MH21(T) and MH72, were isolated from the gut of the earthworms and are proposed as type strains of the novel species Dechloromonas denitrificans sp.
Journal ArticleDOI
Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge
Jie Lu,Yuichi Nogi,Hideto Takami +2 more
TL;DR: It is proposed that the isolate isolated previously from deep-sea sediment collected at a depth of 1050 m on the Iheya Ridge is a member of a new species in a new genus, for which the name Oceanobacillus iheyensis is proposed.
References
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Journal ArticleDOI
Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species
TL;DR: One hundred and nineteen strains of alkaliphilic and alkalitolerant, aerobic endospore-forming bacteria were examined for 47 physiological and biochemical characters, and DNA base composition, and nine of the remainder are proposed as new species.
Journal ArticleDOI
Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov.
TL;DR: The taxonomic positions of six strains of Chryseobacterium meningosepticum and C. miricola were re-evaluated by using a polyphasic taxonomic approach and phylogenetic and phenotypic data showed that they should be transferred to a new genus, Elizabethkingia gen. nov.
Journal ArticleDOI
Taxonomic revision of water-bloom-forming species of oscillatorioid cyanobacteria.
Shoichiro Suda,Makoto M. Watanabe,Shigeto Otsuka,Aparat Mahakahant,Wichien Yongmanitchai,Napavarn Nopartnaraporn,Yongding Liu,John G. Day +7 more
TL;DR: A polyphasic approach was used to clarify the taxonomy of the water-bloom-forming oscillatorioid cyanobacteria and proposed either emended or new taxonomic descriptions for Planktothrix agardhii, PlankTothrix rubescens, and Limnothrix redekei.
Journal ArticleDOI
Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa.
TL;DR: Four N(2)O-producing facultative aerobes, ED1(T), ED5 (T), MH21(T) and MH72, were isolated from the gut of the earthworms and are proposed as type strains of the novel species Dechloromonas denitrificans sp.
Journal ArticleDOI
Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge
Jie Lu,Yuichi Nogi,Hideto Takami +2 more
TL;DR: It is proposed that the isolate isolated previously from deep-sea sediment collected at a depth of 1050 m on the Iheya Ridge is a member of a new species in a new genus, for which the name Oceanobacillus iheyensis is proposed.